Enteric Viruses Group, Murdoch Children's Research Institute, VIC, Australia; Translational Research and Animal Clinical Trial Study (TRACTS) group, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, VIC, Australia.
Enteric Viruses Group, Murdoch Children's Research Institute, VIC, Australia; Department of Pediatrics, The University of Melbourne, VIC, Australia.
Vet Microbiol. 2018 Jul;221:38-43. doi: 10.1016/j.vetmic.2018.05.020. Epub 2018 May 30.
The fecal virome has been investigated in humans and various animal species using next generation sequencing. However, limited information is available about the fecal virome of dogs with chronic enteropathy (CE). We aimed to characterize the canine fecal virome of dogs with CE and compare it with the virome of previously analyzed healthy dogs.A total of 16 adult dogs; 8 healthy dogs (data from a parallel study) and 8 dogs with CE had fecal samples assessed by viral shotgun sequencing. Fecal samples were subjected to enrichment of viral nucleic acids prior to sequencing and metagenomic analyses. Characterization of the complete genome of a canine kobuvirus was performed by Sanger sequencing. An additional 21 healthy dogs and 14 dogs with CE were further analyzed for the prevalence of canine kobuvirus.Three fecal samples from dogs with CE contained in total 3 eukaryotic viral families. In contrast, 4/8 fecal samples previously identified from healthy dogs, contained 5 eukaryotic viral families with 2 families exclusive to this group. Bacteriophages were identified in all fecal samples from CE and healthy dogs. Canine kobuvirus was identified in one dog with CE, by shotgun sequencing, and the complete genome was then characterized. This kobuvirus was classified within canine kobuvirus group, being similar to strains from Korea and China. The larger prevalence study did not detect additional samples positive for canine kobuvirus. The fecal virome of dogs with CE differs in number and type of viral families from healthy dogs. The first Australian canine kobuvirus sequence was identified and characterized from a dog with CE.
采用下一代测序技术研究了人类和各种动物物种的粪便病毒组。然而，关于患有慢性肠炎（CE）的狗的粪便病毒组的信息有限。我们旨在描述患有 CE 的狗的粪便病毒组，并将其与先前分析的健康狗的病毒组进行比较。
总共评估了 16 只成年犬；8 只健康犬（来自平行研究的数据）和 8 只患有 CE 的犬的粪便样本进行了病毒鸟枪法测序。粪便样本在测序和宏基因组分析前进行了病毒核酸富集。通过 Sanger 测序对犬诺如病毒的完整基因组进行了特征描述。进一步对 21 只健康犬和 14 只患有 CE 的犬进行了犬诺如病毒的流行情况分析。
CE 犬的 3 个粪便样本共包含 3 个真核病毒科。相比之下，之前从 8 只健康犬中鉴定出的 4 个粪便样本，包含 5 个真核病毒科，其中 2 个科是该组特有的。在患有 CE 和健康的狗的所有粪便样本中都鉴定出了噬菌体。通过鸟枪法测序在一只患有 CE 的狗中鉴定出了犬诺如病毒，并对其全基因组进行了特征描述。这种诺如病毒被归类为犬诺如病毒组，与来自韩国和中国的菌株相似。更大的流行情况研究没有检测到其他犬诺如病毒阳性样本。患有 CE 的狗的粪便病毒组在病毒家族的数量和类型上与健康狗不同。从患有 CE 的狗中鉴定并特征描述了第一个澳大利亚犬诺如病毒序列。
