Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036, People's Republic of China.
Municipal Key Laboratory of Virology, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, 315010, China.
Arch Virol. 2020 Nov;165(11):2495-2501. doi: 10.1007/s00705-020-04773-6. Epub 2020 Aug 9.
Canine kobuvirus (CaKoV), a newly described virus, is the causative agent of gastroenteritis in dogs. In this study, 57 fecal samples from dogs with diarrhea in Anhui Province, eastern China, were collected. Among these, five samples were identified to be infected with CaKoV, by polymerase chain reaction targeting the CaKoV 3D gene. The five CaKoV strains were subjected to phylogenetic analysis. The sequences of VP1 from the five CaKoV strains were 93.6%-96.1% identical to each other and 91.75%-97.95% identical to other reported CaKoV VP1 sequences. In addition, the complete genome of one strain was successfully amplified and sequenced. The genome consisted of 8223 nucleotides and shared 94.6%-97.0% nucleotide and 93.1%-94.0% amino acid sequence identity with other CaKoV isolates. Phylogenetic analysis revealed that the CaKoV strain from Anhui Province was similar to other Chinese strains, and it was more closely related to feline and mouse kobuviruses than to sheep and bovine kobuviruses. Interestingly, all of the CaKoV-positive samples were coinfected with canine parvovirus. The finding of CaKoV infection in dogs with diarrhea and coinfection with canine parvovirus are a cause for concern and highlight the need for management and preventive measures.
犬诺如病毒（CaKoV）是一种新描述的病毒，是导致犬腹泻的病原体。本研究采集了来自中国东部安徽省 57 份患有腹泻的犬粪便样本。其中，5 份样本通过聚合酶链反应（PCR）检测到 CaKoV 3D 基因被感染。对这 5 株 CaKoV 株进行了系统进化分析。这 5 株 CaKoV 株的 VP1 序列彼此之间的相似度为 93.6%-96.1%，与其他已报道的 CaKoV VP1 序列的相似度为 91.75%-97.95%。此外，还成功扩增并测序了一株的全基因组。该基因组由 8223 个核苷酸组成，与其他 CaKoV 分离株的核苷酸和氨基酸序列相似度分别为 94.6%-97.0%和 93.1%-94.0%。系统进化分析表明，安徽省的 CaKoV 株与其他中国株相似，与猫和鼠诺如病毒的亲缘关系比与绵羊和牛诺如病毒的亲缘关系更密切。有趣的是，所有 CaKoV 阳性样本均与犬细小病毒同时感染。犬腹泻和犬细小病毒同时感染 CaKoV 的发现令人担忧，这突显了需要进行管理和预防措施。
