Department of Biocatalysis. Institute of Catalysis and Petrochemistry (ICP), CSIC, Campus UAM, Cantoblanco, 28049 Madrid, Spain.
Department of Biocatalysis. Institute of Catalysis and Petrochemistry (ICP), CSIC, Campus UAM, Cantoblanco, 28049 Madrid, Spain; Pharmacy and Biotechnology Department, School of Biomedical Sciences, Universidad Europea, Madrid, Spain.
Bioresour Technol. 2018 Oct;266:249-258. doi: 10.1016/j.biortech.2018.06.070. Epub 2018 Jun 22.
The present study focuses on the development and optimization of a packed-bed reactor (PBR) for continuous production of xylooligosaccharides (XOS) from xylan. For this purpose, three different methacrylic polymer-based supports (Relizyme R403/S, Purolite P8204F and Purolite P8215F) activated with glyoxyl groups were morphologically characterized and screened for the multipoint covalent immobilization of a xylanase. Based on its physical and mechanical properties, maximum protein loading and thermal stability, Relizyme R403/S was selected to set up a PRB for continuous production of XOS from corncob xylan. The specific productivity for XOS at 10 mL/min flow rate was 3277 g g h with a PBR. This PBR conserved >90% of its initial activity after 120 h of continuous operation.
本研究专注于开发和优化填充床反应器（PBR），以从木聚糖连续生产木低聚糖（XOS）。为此，使用三种不同的甲基丙烯酰基聚合物基载体（Relizyme R403/S、Purolite P8204F 和 Purolite P8215F），用乙二醛基团进行了形态学表征，并筛选出多点共价固定木聚糖酶。基于其物理和机械性能、最大蛋白质负载量和热稳定性，选择 Relizyme R403/S 来建立填充床反应器，从玉米芯木聚糖连续生产 XOS。在 10 mL/min 的流速下，XOS 的比生产力为 3277 g g h，填充床反应器的保留率为初始活性的 >90%。在 120 小时的连续运行后，该 PBR 仍保留了 >90%的初始活性。
