Department of Chemistry, McGill University, 801 Sherbrooke Street West, Montreal, QC H3A 0B8, Canada.
Bioresource Engineering Department, McGill University, 21111 Lakeshore Road, Ste-Anne de Bellevue, QC H9X 3V9, Canada.
Molecules. 2019 Nov 20;24(23):4206. doi: 10.3390/molecules24234206.
Current enzymatic methods for hemicellulosic biomass depolymerization are solution-based, typically require a harsh chemical pre-treatment of the material and large volumes of water, yet lack in efficiency. In our study, xylanase (E.C. 3.2.1.8) from is used to hydrolyze xylans from different sources. We report an innovative enzymatic process which avoids the use of bulk aqueous, organic or inorganic solvent, and enables hydrolysis of hemicellulose directly from chemically untreated biomass, to low-weight, soluble oligoxylosaccharides in >70% yields.
目前用于半纤维素生物质解聚的酶法是基于溶液的，通常需要对材料进行苛刻的化学预处理和大量的水，但效率低下。在我们的研究中，来自 的木聚糖酶（E.C. 3.2.1.8）用于水解来自不同来源的木聚糖。我们报告了一种创新的酶法工艺，它避免了使用大量的水、有机溶剂或无机溶剂，并能够直接从未经化学处理的生物质中水解半纤维素，生成>70%收率的低分子量、可溶性寡木糖。
