College of Chemistry and Chemical Engineering, State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, PR China.
College of Chemistry and Chemical Engineering, State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Nov 5;204:641-647. doi: 10.1016/j.saa.2018.06.076. Epub 2018 Jun 21.
In this study, a novel fluorescent probe, 6‑hydroxychromone‑3‑carbaldehyde‑(rhodamine B carbonyl) hydrazine (L), for Zn and Al was designed and synthesized. Initially, this probe L exhibited inferior fluorescence emission peak centered at 488 nm in EtOH/HEPES solution (3/1, 10.0 μM HEPES, pH 7.4) when excited at 421 nm. After the addition of Zn, this probe L displayed excellent selectivity towards Zn with obvious fluorescence color change from colorless to yellow, which might be attributed to the formation of a 1:1 ligand-metal complex resulting in the inhibition of photo-induced electron transfer phenomenon. Whereas, the prepared Zn complex of L could be used as a ratiometric fluorescent probe to detect Al on the basis of fluorescence resonance energy transfer mechanism. This ligand-metal complex of Zn (LZn) showed high selectivity towards Al with obvious enhancement in fluorescence emission intensity at 580 nm and remarkable decrease in fluorescence emission intensity at 488 nm, and the fluorescence color also changed from yellow to pink. Furthermore, the detection limit of the probe L, LZn towards Zn, Al were 1.25 × 10 M and 3.179 × 10 M, respectively. Additionally, the complexation properties of L towards Zn and LZn towards Al were studied in detail.
在这项研究中，设计并合成了一种用于 Zn 和 Al 的新型荧光探针，6-羟基香豆素-3-甲醛-（若丹明 B 羰基）腙（L）。最初，该探针 L 在 421nm 激发下，在 EtOH/HEPES 溶液（3/1，10.0μM HEPES，pH7.4）中显示出中心位于 488nm 的较差荧光发射峰。加入 Zn 后，探针 L 对 Zn 表现出优异的选择性，荧光颜色从无色变为黄色，这可能归因于形成 1:1 配体-金属配合物，从而抑制光致电子转移现象。而 L 的制备的 Zn 配合物可以基于荧光共振能量转移机制用作比率荧光探针来检测 Al。Zn（LZn）的这种配体-金属配合物对 Al 表现出高选择性，在 580nm 处的荧光发射强度明显增强，在 488nm 处的荧光发射强度明显降低，荧光颜色也从黄色变为粉红色。此外，探针 L、LZn 对 Zn、Al 的检测限分别为 1.25×10-5M 和 3.179×10-5M。此外，还详细研究了 L 对 Zn 的络合性质和 LZn 对 Al 的络合性质。
