基于苯并噻唑的荧光探针用于 Al 的比率型检测及其在水样和细胞成像中的应用。

Department of Applied Chemistry, College of Arts and Sciences, Northeast Agricultural University, Harbin 150030, China.

Int J Mol Sci. 2019 Nov 28;20(23):5993. doi: 10.3390/ijms20235993.

An easily prepared benzothiazole-based probe (BHM) was prepared and characterized by general spectra, including H NMR, C NMR, HRMS, and single-crystal X-ray diffraction. Based on the synergistic mechanism of the inhabitation of intramolecular charge transfer (ICT), the BHM displayed high selectivity and sensitivity for Al in DMF/HO (, 1/1) through an obvious blue-shift in the fluorescent spectrum and significant color change detected by the naked eye, respectively. The binding ratio of BHM with Al was 1:1, as determined by the Job plot, and the binding details were investigated using FT-IR, H NMR titration, and ESI-MS analysis. Furthermore, the BHM was successfully applied in the detection of Al in the Songhua River and on a test stripe. Fluorescence imaging experiments confirmed that the BHM could be used to monitor Al in human stromal cells (HSC).

一种易于制备的苯并噻唑基探针（BHM）通过包括 H NMR、C NMR、HRMS 和单晶 X 射线衍射在内的常规光谱进行了制备和表征。基于抑制分子内电荷转移（ICT）的协同机制，BHM 在 DMF/HO（1/1，体积比）中对 Al 表现出高选择性和灵敏度，荧光光谱明显蓝移，肉眼可检测到明显的颜色变化。通过 Job 图确定 BHM 与 Al 的结合比为 1:1，并通过 FT-IR、H NMR 滴定和 ESI-MS 分析研究了结合细节。此外，BHM 成功应用于松花江和测试条中 Al 的检测。荧光成像实验证实，BHM 可用于监测人基质细胞（HSC）中的 Al。