Salem Neveen A, Hamza Amal, Alnahdi Hanan, Ayaz Najla
Applied Biochemistry Department, Faculty of Science, University of Jeddah, Jeddah, Saudi Arabia.
Narcotics, Ergogenic Aids and Poisons Department, National Research Centre, Giza, Egypt.
Cell Physiol Biochem. 2018;47(6):2331-2339. doi: 10.1159/000491544. Epub 2018 Jul 6.
BACKGROUND/AIMS: Hepatic fibrosis is a wound-healing process in the chronically injured liver. Clinical application of platelet-rich plasma (PRP) is of considerable interest for wound healing and regeneration. In view of the regeneration effect of PRP, we designed this study to explore the hypothesis that PRP could play a role in improving the biochemical and molecular changes that occur in liver fibrosis induced by dimethylnitrosamine (DMN) in rats.
Four groups were studied: control, PRP control, DMN (liver fibrosis), and DMN+PRP groups. Serum liver enzymes (alanine amino transferase ALT, aspartate amino transferase AST, gamma glutamyl transferase GGT, and lactate dehydrogenase LDH), and liver hydroxyproline content were measured colorimetrically.Interleukin-8 (IL-8) and B-cell lymphoma (Bcl2) were determined by enzyme-linked immunosorbent assay. And the expression levels of alpha-smooth muscle actin (α-SMA) ,transforming growth factor (TGF-β), and nuclear factor kappa B1(NF-қB1) were evaluated by quantitative real-time polymerase chain reaction.
Our results showed that PRP markedly improved the DMN-induced changes in liver enzymes accompanied by a significant decrease in liver hydroxyproline content and IL-8 level induced by DMN, and an increase in the anti-apoptotic marker Bcl-2. PRP also showed significant down-regulation of fibrosis-related genes α-SMA and TGF-β and a significant decrease in the inflammatory marker NF-қB1.
Based on these encouraging results, we consider that PRP could be a promising new agent for liver regeneration and alleviation of fibrosis.
背景/目的：肝纤维化是慢性损伤肝脏的一种伤口愈合过程。富含血小板血浆（PRP）在伤口愈合和再生方面的临床应用备受关注。鉴于PRP的再生作用，我们设计了本研究，以探讨PRP是否能改善大鼠二甲基亚硝胺（DMN）诱导的肝纤维化中发生的生化和分子变化这一假说。
研究分为四组：对照组、PRP对照组、DMN（肝纤维化）组和DMN + PRP组。采用比色法测定血清肝酶（丙氨酸氨基转移酶ALT、天冬氨酸氨基转移酶AST、γ-谷氨酰转移酶GGT和乳酸脱氢酶LDH）以及肝脏羟脯氨酸含量。通过酶联免疫吸附测定法测定白细胞介素-8（IL-8）和B细胞淋巴瘤（Bcl2）。并通过定量实时聚合酶链反应评估α-平滑肌肌动蛋白（α-SMA）、转化生长因子（TGF-β）和核因子κB1（NF-қB1）的表达水平。
我们的结果表明，PRP显著改善了DMN诱导的肝酶变化，同时DMN诱导的肝脏羟脯氨酸含量和IL-8水平显著降低，抗凋亡标志物Bcl-2增加。PRP还显示出纤维化相关基因α-SMA和TGF-β的显著下调以及炎症标志物NF-қB1的显著降低。
基于这些令人鼓舞的结果，我们认为PRP可能是一种有前途的肝脏再生和减轻纤维化的新型药物。
