Universidad Autónoma de Nuevo León, Centro de Investigación en Nutrición y Salud Pública, Monterrey, NL, México.
Biochemical Sciences Division, CSIR-National Chemical Laboratory, Pune-411008, India.
FEMS Yeast Res. 2018 Dec 1;18(8). doi: 10.1093/femsyr/foy073.
Yarrowia lipolytica is an ascomycetous dimorphic yeast with immense potential for industrial applications, including bioremediation of crude oil-contaminated environments. It has been shown that a dimorphic marine isolate of Y. lipolytica (var. indica) has significant capacity to degrade fatty acids and alkanes, when in its yeast morphology. It has also been demonstrated that polyamines play an important role in the yeast-to-mycelium transition of different strains of Y. lipolytica that are unable to utilize those carbon sources. To determine the role of polyamines on their capacity to utilize oils and hydrocarbons, on the dimorphic transition, and also on other characteristics of the var. indica strain of Y. lipolytica, we proceeded to obtain ornithine decarboxylase minus (odc-) mutants. These mutants behaved as yeasts independently of the concentrations of putrescine added. Further, they conserved the oil-degrading capacity of the parent strain. The odc- mutant can thus be used in fatty acid degradation, and oil spill remediation with distinct advantages.
解脂耶氏酵母是一种有性二态真菌，具有巨大的工业应用潜力，包括生物修复受原油污染的环境。已经表明，解脂耶氏酵母（var. indica）的一种二态海洋分离株在其酵母形态时具有显著的降解脂肪酸和烷烃的能力。还已经证明，多胺在不能利用这些碳源的不同解脂耶氏酵母菌株的酵母-菌丝体转变中起着重要作用。为了确定多胺对其利用油脂和碳氢化合物的能力、二态转变以及解脂耶氏酵母 var. indica 菌株的其他特性的作用，我们继续获得鸟氨酸脱羧酶缺失（odc-）突变体。这些突变体在添加腐胺的浓度下独立表现为酵母。此外，它们保留了亲本菌株的油脂降解能力。因此，odc- 突变体可用于脂肪酸降解和溢油修复，具有明显的优势。
