Department of Medical Microbiology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
J Antimicrob Chemother. 2018 Oct 1;73(10):2687-2690. doi: 10.1093/jac/dky236.
In this study we assess the antibiotic resistance genes in three metronidazole-resistant Prevotella bivia clinical isolates.
Strains were whole-genome sequenced. De novo assembly was performed and genes were annotated in RAST. Manual adjustments were made, when required, to the annotation and length of the genes.
In all three strains a novel nim gene, nimK, was encountered located on a mobile genetic element (MGE). The nimK gene was associated with an IS1380 family transposase. On the same MGE, genes encoding an efflux small MDR (SMR) transporter were present and were associated with a crp/fnr regulator.
This is the first description of the presence of a novel nim gene in metronidazole-resistant P. bivia clinical isolates. This gene is co-located with an efflux SMR transporter on an MGE, which has been named Tn6456 (MG827401). The identification of these resistance genes on an MGE is worrisome, since this indicates the horizontal gene transfer of antibiotic and/or biocide resistance from one strain to the other.
本研究评估了三株甲硝唑耐药卟啉单胞菌临床分离株中的抗生素耐药基因。
对菌株进行全基因组测序。在 RAST 中进行从头组装和基因注释。当需要时，对基因的注释和长度进行手动调整。
在所有三株菌中，均遇到了位于移动遗传元件（MGE）上的新型 nim 基因 nimK。nimK 基因与 IS1380 家族转座酶相关。在同一个 MGE 上，存在编码外排小多药耐药（SMR）转运蛋白的基因，并与 crp/fnr 调节剂相关。
这是首次描述甲硝唑耐药卟啉单胞菌临床分离株中存在新型 nim 基因。该基因与 MGE 上的外排 SMR 转运蛋白共定位，该 MGE 被命名为 Tn6456（MG827401）。在 MGE 上鉴定出这些耐药基因令人担忧，因为这表明抗生素和/或杀生物剂耐药性从一株菌向另一株菌的水平基因转移。
