SugE，SMR 家族转运蛋白的新成员，有助于阴沟肠杆菌的抗菌药物耐药性。
SugE, a new member of the SMR family of transporters, contributes to antimicrobial resistance in Enterobacter cloacae.
机构信息
Department of Clinical Laboratory and Nutritional Sciences, University of Massachusetts Lowell, Lowell, MA 01854, USA.
出版信息
Antimicrob Agents Chemother. 2011 Aug;55(8):3954-7. doi: 10.1128/AAC.00094-11. Epub 2011 May 16.
Abstract
We cloned a gene, sugE, from the chromosome of Enterobacter cloacae ATCC 13047. Analysis of the susceptibilities of the sugE-containing strain (Escherichia coli KAM32/pSUGE28) and sugE-deficient E. cloacae (EcΔsugE) showed that SugE confers resistance to cetyltrimethylammonium bromide, cetylpyridinium chloride, tetraphenylphosphonium, benzalkonium chloride, ethidium bromide, and sodium dodecyl sulfate. We also investigated expression of sugE. We confirm here that SugE from E. cloacae is an SMR family transporter as determined by observing its energy-dependent drug efflux activity.
摘要
我们从肠杆菌 cloacae ATCC 13047 的染色体中克隆了一个 sugE 基因。对含 sugE 菌株（大肠杆菌 KAM32/pSUGE28）和 sugE 缺陷型肠杆菌 cloacae（EcΔsugE）的药敏分析表明，SugE 赋予了它们对十六烷基三甲基溴化铵、十六烷基氯化吡啶、四苯膦、苯扎氯铵、溴化乙锭和十二烷基硫酸钠的抗性。我们还研究了 sugE 的表达情况。我们在这里证实，来自肠杆菌 cloacae 的 SugE 是一种 SMR 家族转运蛋白，这是通过观察其能量依赖性药物外排活性来确定的。
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Biol Pharm Bull. 2008 Mar;31(3):516-9. doi: 10.1248/bpb.31.516.
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