Braus G, Furter R, Prantl F, Niederberger P, Hütter R
Arch Microbiol. 1985 Sep;142(4):383-8. doi: 10.1007/BF00491908.
The tryptophan biosynthetic genes TRP1 and TRP3 and partly also TRP2 and TRP4 have been compared by the technique of Southern hybridization and enzyme measurements in twelve wild isolates of Saccharomyces cerevisiae from natural sources of different continents, in the commonly used laboratory strain S. cerevisiae X2180-1A and in a Kluyveromyces marxianus strain. We could classify these strains into four groups, which did not correlate with their geographical distribution. In no case are the TRP3 and TRP1 genes fused as has been found in other ascomycetes. Two strains were found which, in contrast to strain X2180-1A, show derepression of gene TRP1. Two examples are discussed to demonstrate the usefulness of Southern hybridizations for the identification of closely related strains.
通过Southern杂交技术和酶活性测定，对来自不同大陆自然来源的12株酿酒酵母野生分离株、常用的实验室菌株酿酒酵母X2180-1A和一株马克斯克鲁维酵母菌株中的色氨酸生物合成基因TRP1和TRP3，以及部分TRP2和TRP4进行了比较。我们可以将这些菌株分为四组，这与它们的地理分布无关。在任何情况下，TRP3和TRP1基因都没有像在其他子囊菌中那样融合。发现有两株菌株与X2180-1A菌株不同，它们表现出TRP1基因的去阻遏。讨论了两个例子以证明Southern杂交在鉴定密切相关菌株方面的有用性。
