Irniger S, Egli C M, Braus G H
Institute of Microbiology, Swiss Federal Institute of Technology (ETH), Zürich.
Curr Genet. 1993 Mar;23(3):201-4. doi: 10.1007/BF00351496.
We have tested the functioning of the human c-myc polyadenylation signal in Saccharomyces cerevisiae. A DNA fragment containing the two AATAAA polyadenylation signals of the c-myc gene was inserted into a plasmid designed for the in-vivo testing of polyadenylation signals in yeast. The c-myc fragment had a partial capacity for directing mRNA 3'-end formation in yeast. The 3'-endpoints were 50-100 bp distant from the mRNA 3'-ends mapped in humans. This human DNA fragment is therefore unspecifically functional in yeast, indicating that other sequence elements than the human polyadenylation signal, AATAAA, are necessary for 3'-end formation.
我们已经在酿酒酵母中测试了人类c-myc聚腺苷酸化信号的功能。将包含c-myc基因两个AATAAA聚腺苷酸化信号的DNA片段插入到一个设计用于在酵母中进行聚腺苷酸化信号体内测试的质粒中。c-myc片段在酵母中具有部分指导mRNA 3'端形成的能力。3'端点与在人类中定位的mRNA 3'端相距50-100 bp。因此，这个人类DNA片段在酵母中具有非特异性功能，这表明除了人类聚腺苷酸化信号AATAAA之外，其他序列元件对于3'端形成也是必需的。
