Department of Veterinary Sciences, Federal University of Parana - UFPR, Rua Pioneiro, 2153, 85950-000, Palotina, Paraná, Brazil.
Department of Preventive Veterinary Medicine, State University of Londrina - UEL, PO Box 6001, 86051-990, Londrina, Paraná, Brazil.
Virus Res. 2018 Aug 15;255:10-13. doi: 10.1016/j.virusres.2018.06.016. Epub 2018 Jul 3.
This study reports the detection by RT-PCR and molecular characterization of partial RdRp gene of picobirnavirus (PBV) dsRNA in fecal samples (n = 100) from a meat sheep flock in southern Brazil. The analysis of the results allowed the identification of two important characteristics of PBV infection. The first was the high frequency of infection in the sheep flock evaluated where 62% of the analyzed fecal samples were PBV-positive. The second was the high genetic variability found in field strains of ovine PBV genogroup I circulating in animals of the same sheep flock.
本研究通过 RT-PCR 法检测并对巴西南部肉绵羊群粪便样本（n=100）中的小双 RNA 病毒（PBV）dsRNA 的 RdRp 基因的部分序列进行了分子特征分析。对结果的分析鉴定了 PBV 感染的两个重要特征。首先是在评估的绵羊群中感染的高频率，在所分析的粪便样本中，62%为 PBV 阳性。其次是在同一绵羊群动物中流行的 I 型羊 PBV 基因型的田间毒株中发现的高遗传变异性。
