Karayel-Hacioglu Ilke, Gul Buket, Alkan Feray
Department of Virology, Faculty of Veterinary Medicine, Ankara University, Diskapi, 06110, Ankara, Turkey.
Department of Virology, Graduate School of Health Sciences, Ankara University, Sehit Omer Halisdemir Street, Diskapi, 06110, Ankara, Turkey.
Virus Genes. 2022 Jun;58(3):238-243. doi: 10.1007/s11262-022-01894-9. Epub 2022 Mar 15.
Picobirnaviruses (PBVs), detected in a wide range of host species, are viruses of which limited information is available about their pathogenic potential, ecology, or evolutionary characteristics. In this study, a molecular analysis of segment 2 encoding the PBV RNA-dependent RNA-polymerase (RdRp) in small ruminants with diarrhea in Turkey was undertaken. A total of 66 fecal samples or gut contents from diarrheic small ruminants including 55 sheep and 11 goats were screened. Four samples (6.06%), obtained from sheep in different farms, yielded the expected amplicon size for the genogroup I RdRp gene fragment, whereas no positivity was detected for genogroup II PBVs. Phylogenetic analysis revealed high levels of genetic diversity among the genogroup I PBVs. Additionally, all PBV infected sheep were also positive for rotavirus A. This study, reporting the presence of the PBVs in sheep Turkey for the first time, contributes to the molecular characterization and epidemiology of PBVs.
微小双股RNA病毒（PBV）在多种宿主物种中均有发现，然而，关于其致病潜力、生态学或进化特征的信息却极为有限。本研究针对土耳其患有腹泻的小反刍动物中编码PBV RNA依赖性RNA聚合酶（RdRp）的第2节段进行了分子分析。总共对66份来自腹泻小反刍动物的粪便样本或肠道内容物进行了筛查，其中包括55只绵羊和11只山羊。从不同农场的绵羊中获取的4份样本（6.06%），扩增出了与基因群I RdRp基因片段预期大小相符的条带，而基因群II PBV未检测到阳性结果。系统发育分析显示，基因群I PBV之间存在高度的遗传多样性。此外，所有感染PBV的绵羊同时也感染了A组轮状病毒。本研究首次报道了土耳其绵羊中存在PBV，为PBV的分子特征及流行病学研究提供了依据。
