Druse M J, Kelly G M
Alcohol. 1985 Sep-Oct;2(5):667-70. doi: 10.1016/0741-8329(85)90144-2.
The activity of Mg2+-activated, ouabain-sensitive adenosine triphosphatase, (Na+-K+)-ATPase, was determined in homogenates of hypothalamus, cortex, cerebellum, and brain stem from the 19-day-old offspring of rats that were pair-fed control or (6.6%, v/v) ethanol liquid diets on a chronic basis prior to parturition. In the offspring of both control and ethanol-fed rats the specific activity of (Na+-K+)-ATPase was significantly (p less than 0.01) greater in the cortex than it was in the hypothalamus, brain stem or cerebellum (hypothalamus approximately brain stem approximately cerebellum). When the offspring of ethanol-fed and control rats were compared we observed no significant (p greater than 0.05) differences in the activity of (Na+-K+)-ATPase in any of the four brain regions examined. In addition, the results of kinetic analyses of cortical (Na+-K+)-ATPase were similar in the 19-day-old offspring of ethanol-fed rats and those whose mothers consumed either the control liquid diet or standard laboratory chow. The results of these studies suggest that the activity of the plasma membrane enzyme, (Na+-K+)-ATPase, was not affected in the 19-day-old offspring of ethanol-fed rats.
测定了在分娩前长期成对喂饲对照或（6.6%，v/v）乙醇液体饲料的19日龄大鼠后代的下丘脑、皮层、小脑和脑干匀浆中镁离子激活的、哇巴因敏感的三磷酸腺苷酶（钠钾ATP酶）的活性。在对照和乙醇喂养大鼠的后代中，皮层中钠钾ATP酶的比活性显著（p<0.01）高于下丘脑、脑干或小脑（下丘脑≈脑干≈小脑）。当比较乙醇喂养和对照大鼠的后代时，我们发现在所检测的四个脑区中，钠钾ATP酶的活性没有显著（p>0.05）差异。此外，在乙醇喂养大鼠的19日龄后代以及其母亲食用对照液体饲料或标准实验室饲料的后代中，皮层钠钾ATP酶的动力学分析结果相似。这些研究结果表明，在乙醇喂养大鼠的19日龄后代中，质膜酶钠钾ATP酶的活性未受影响。
