Departments of Craniofacial Sciences, Division of Pediatric Dentistry, School of Dental Medicine, University of Connecticut Health Center, Farmington, CT, USA.
Int Endod J. 2019 Jan;52(1):68-76. doi: 10.1111/iej.12983. Epub 2018 Jul 26.
To examine the contribution of perivascular cells expressing αSMA to reactionary dentinogenesis.
An inducible, Cre-loxP in vivo fate-mapping approach was used to examine the contribution of the descendants of cells expressing the αSMA-CreERT2 transgene to reactionary dentinogenesis in mice molars. Reactionary dentinogenesis was induced by experimental mild injury to dentine without pulp exposure. The Student's t test was used to determine statistical significance at *P ≤ 0.05.
The lineage tracing experiments revealed that mild injury to dentine first led to activation of αSMA-tdTomato cells in the entire pulp chamber. The percentage of areas occupied by αSMA-tdTomato in injured (7.5 ± 0.7%) teeth were significantly higher than in teeth without injury (2 ± 0.5%). After their activation, αSMA-tdTomato cells migrated towards the site of injury, gave rise to pulp cells and a few odontoblasts that became integrated into the existing odontoblast layer expressing Col2.3-GFP and Dspp.
Mild insult to dentine activated perivascular αSMA-tdTomato cells giving rise to pulp cells as well as a few odontoblasts that were integrated into the pre-existing odontoblast layer.
研究表达αSMA 的血管周细胞对反应性牙本质形成的贡献。
采用诱导型、Cre-loxP 体内示踪方法，研究表达αSMA-CreERT2 转基因的细胞后代对小鼠磨牙反应性牙本质形成的贡献。通过对牙髓无暴露的牙本质进行轻度损伤来诱导反应性牙本质形成。采用 Student's t 检验确定 *P≤0.05 时的统计学意义。
谱系追踪实验表明，牙本质的轻度损伤首先导致整个牙髓腔内的αSMA-tdTomato 细胞被激活。损伤（7.5±0.7%）牙齿中αSMA-tdTomato 细胞所占面积的百分比明显高于未损伤（2±0.5%）牙齿。激活后，αSMA-tdTomato 细胞向损伤部位迁移，产生牙髓细胞和少数成牙本质细胞，这些细胞整合到表达 Col2.3-GFP 和 Dspp 的现有成牙本质细胞层中。
牙本质的轻度损伤激活了血管周的αSMA-tdTomato 细胞，产生了牙髓细胞和少数成牙本质细胞，这些细胞整合到预先存在的成牙本质细胞层中。
