1 Departments of Craniofacial Sciences, School of Dental Medicine, University of Connecticut Health Center, Farmington, CT, USA.
2 John A. Paulson School of Engineering and Applied Sciences and Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, MA, USA.
J Dent Res. 2018 Sep;97(10):1170-1177. doi: 10.1177/0022034518769827. Epub 2018 Apr 12.
The goal of this study was to examine the effects of early and limited exposure of perivascular cells expressing α (αSMA) to fibroblast growth factor 2 (FGF2) in vivo. We performed in vivo fate mapping by inducible Cre-loxP and experimental pulp injury in molars to induce reparative dentinogenesis. Our results demonstrate that early delivery of exogenous FGF2 to exposed pulp led to proliferative expansion of αSMA-tdTomato cells and their accelerated differentiation into odontoblasts. In vivo lineage-tracing experiments showed that the calcified bridge/reparative dentin in FGF2-treated pulps were lined with an increased number of Dspp odontoblasts and devoid of BSP osteoblasts. The increased number of odontoblasts derived from αSMA-tdTomato cells and the formation of reparative dentin devoid of osteoblasts provide in vivo evidence for the stimulatory effects of FGF signaling on odontoblast differentiation from early progenitors in dental pulp.
本研究的目的是探讨体内表达α（αSMA）的血管周细胞早期和有限暴露于成纤维细胞生长因子 2（FGF2）的影响。我们通过诱导性 Cre-loxP 以及磨牙内的实验性牙髓损伤进行体内命运图谱分析，以诱导修复性牙本质形成。我们的结果表明，早期将外源性 FGF2 递送至暴露的牙髓会导致 αSMA-tdTomato 细胞的增殖扩张，并加速其分化为成牙本质细胞。体内谱系追踪实验表明，在 FGF2 处理的牙髓中，钙化桥/修复性牙本质内衬有数量增加的 Dspp 成牙本质细胞，而缺乏 BSP 成骨细胞。源自 αSMA-tdTomato 细胞的成牙本质细胞数量增加以及无成骨细胞形成的修复性牙本质为 FGF 信号对牙髓中早期祖细胞的成牙本质细胞分化的刺激作用提供了体内证据。
