Henry H L, Norman A W
Calcif Tissue Int. 1985 Sep;37(5):484-90. doi: 10.1007/BF02557831.
The effects of intraperitoneal injections of aluminum chloride were tested on the intestinal calcium absorption and bone calcium mobilization responses to vitamin D3 and 1,25(OH)2D3, as measured by bioassay in chicks. Aluminum at 5 mg/kg given 5 days before the bioassay in vitamin D-deficient chicks, partially blocked the intestinal calcium absorption response to low (0.65 and 3.2 nmol), but not to higher (32 nmol) doses of vitamin D3. The responses to all doses (0.32-2.1 nmol) of 1,25(OH)2D3 were partially blocked by aluminum treatment. Serum calcium values were elevated in vitamin D-deficient chicks by aluminum administration, but no consistent effects of the treatment on bone calcium mobilization in response to vitamin D3 or 1,25(OH)2D3 were noted. Aluminum treatment in vivo led to decreased 25-OH-D3-1-hydroxylase activity subsequently measured in renal homogenates; under a variety of conditions, no direct effect of aluminum on 25-OH-D3 metabolism by primary cultures of chick kidney cells was observed. The results suggest that the ability of the intestine to respond normally to 1,25(OH)2D3 may be compromised by exposure to high levels of aluminum and that the effect of this element on 25-OH-D3 metabolism observed in vivo may not be exerted by direct action on the renal cell.
通过对雏鸡进行生物测定，研究了腹腔注射氯化铝对肠道钙吸收以及对维生素D3和1,25(OH)2D3的骨钙动员反应的影响。在维生素D缺乏的雏鸡进行生物测定前5天给予5mg/kg的铝，部分阻断了肠道对低剂量（0.65和3.2nmol）维生素D3的钙吸收反应，但对高剂量（32nmol）维生素D3没有影响。铝处理部分阻断了对所有剂量（0.32 - 2.1nmol）1,25(OH)2D3的反应。给予铝可使维生素D缺乏的雏鸡血清钙值升高，但未观察到该处理对维生素D3或1,25(OH)2D3引起的骨钙动员有一致影响。体内铝处理导致随后在肾匀浆中测得的25 - OH - D3 - 1 - 羟化酶活性降低；在各种条件下，未观察到铝对雏鸡肾细胞原代培养物中25 - OH - D3代谢的直接影响。结果表明，暴露于高水平铝可能会损害肠道对1,25(OH)2D3正常反应的能力，并且在体内观察到的该元素对25 - OH - D3代谢的影响可能不是通过对肾细胞的直接作用来发挥的。
