Department of Life Science, College of Natural Sciences, Hanyang University, Seoul 04763, Korea.
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
Int J Mol Sci. 2018 Jul 6;19(7):1969. doi: 10.3390/ijms19071969.
has great potential as a bio-factory for production of value-added compounds. To produce the desired chemicals more efficiently in , genetic tools for modification of need to be developed, especially an endogenous promoter. In this study, the promoter of photosystem I protein D () from UTEX395 was identified. Computational analysis revealed the presence of several putative cis-acting elements, including a potential core element, and light-responsive or stress-responsive elements. Gene expression analysis in heterologous expression system in and showed that promoter can be used to drive the expression of genes. Functional analysis of this promoter suggested that the initiator element (Inr) is important for its function (i.e., TATA-less promoter) and that an additional factor (e.g., downstream of the transcriptional start site) might be needed for light response. We have shown that the promoter is functional, but not sufficiently strong, both in microalgae and higher plant.
具有作为生产增值化合物的生物工厂的巨大潜力。为了更有效地在 中生产所需的化学品，需要开发用于修饰 的遗传工具，特别是内源性启动子。在这项研究中，从 UTEX395 中鉴定出了光系统 I 蛋白 D ()的启动子。计算分析显示存在几个假定的顺式作用元件，包括一个潜在的核心元件，以及光响应或应激响应元件。在 和 中的异源表达系统中的基因表达分析表明， 启动子可用于驱动基因的表达。该启动子的功能分析表明，起始元件 (Inr) 对于其功能（即 TATA-less 启动子）很重要，并且可能需要额外的因子（例如，在转录起始位点的下游）来进行光响应。我们已经表明， 启动子在微藻和高等植物中均具有功能，但不够强。
