Research Center for Applied Sciences, Academia Sinica, Taipei 11529, Taiwan.
Institute of Biophotonics, National Yang-Ming University, Taipei 11221, Taiwan.
Sensors (Basel). 2018 Jul 6;18(7):2181. doi: 10.3390/s18072181.
A spectral integration combined with a threshold method for the analysis of spectral scanning surface plasmon resonance (SPR) images can significantly increase signal recognition at low concentration of antibody solution. The 12-well SPR sensing plates consisted of gold-capped nanowire arrays with 500-nm period, 80-nm linewidth and 50-nm gold thickness which were used for generating multiple SPR images. A threshold method is introduced to eliminate background noises in spectral scanning images. Combining spectral integration and the threshold method, the detection limit of antibody concentration was 1.23 ng/mL. Using multiple-well SPR sensing plates and the proposed analytical method, multiple kinetic responses with spectral and spatial information on different sensing areas can be sensitively measured.
光谱积分与阈值法相结合用于分析光谱扫描表面等离子体共振（SPR）图像，可以显著提高低浓度抗体溶液中的信号识别能力。12 孔 SPR 传感板由具有 500nm 周期、80nm 线宽和 50nm 金厚的金纳米线阵列组成，用于产生多个 SPR 图像。阈值法用于消除光谱扫描图像中的背景噪声。结合光谱积分和阈值法，抗体浓度的检测限为 1.23ng/mL。使用多井 SPR 传感板和所提出的分析方法，可以敏感地测量不同传感区域的具有光谱和空间信息的多个动力学响应。
