Kurata A, Katamine S, Fukuda T, Mine M, Ikari N, Kanazawa H, Matsunaga M, Eguchi K, Nagataki S
Clin Exp Immunol. 1985 Oct;62(1):65-74.
Human T-cell leukaemia virus (HTLV1/ATLV), which causes adult T cell leukaemia (ATL), is an infectious, lymphotrophic retrovirus unique for humans. The present study was undertaken to determine whether HTLV1 had any pathogenetic role for systemic lupus erythematosus (SLE). The incidence of antibodies to ATL cell-associated antigens (ATLA) in sera from patients with SLE and other collagen diseases was investigated by an indirect immunofluorescent cytoplasmic staining of an HTLV1-infected cell line (MT-1). A radioimmunoassay was also performed to detect antibodies to HTLV1 protein and crude membrane fraction derived from an HTLV1-producing cell line MT-2. Furthermore, an Epstein-Barr virus (EBV)-transformed B cell line (ES-1) was constructed from an SLE patient, which produced a monoclonal antibody (IgG, lambda) reactive to an HTLV1-related cell-membrane antigen expressed on MT-1 and MT-2 cells. The specific reactivity of the monoclonal antibody was analysed by an indirect immunofluorescent cell-membrane staining and a microcytotoxicity test. The incidence of anti-ATLA antibodies was not different among SLE and other collagen diseases. The monoclonal antibody produced by ES-1 stained and killed HTLV1-infected cell lines specifically, but did not react with other human lymphoid cell lines. This monoclonal antibody failed to react with peripheral blood mononuclear cells (PBMC), mitogen-induced T cell blasts, and iododeoxyuridine-treated T cells from SLE patients. Thus, a possible role of HTLV1 in the aetiology of SLE was not established.
人类T细胞白血病病毒（HTLV1/ATLV）可引发成人T细胞白血病（ATL），是一种人类特有的传染性嗜淋巴细胞逆转录病毒。本研究旨在确定HTLV1对系统性红斑狼疮（SLE）是否具有任何致病作用。通过对HTLV1感染的细胞系（MT-1）进行间接免疫荧光细胞质染色，研究了SLE患者和其他胶原病患者血清中抗ATL细胞相关抗原（ATLA）抗体的发生率。还进行了放射免疫测定，以检测抗HTLV1蛋白抗体和源自产生HTLV1的细胞系MT-2的粗膜部分。此外，从一名SLE患者构建了一种爱泼斯坦-巴尔病毒（EBV）转化的B细胞系（ES-1），该细胞系产生了一种对MT-1和MT-2细胞上表达的HTLV1相关细胞膜抗原具有反应性的单克隆抗体（IgG，λ）。通过间接免疫荧光细胞膜染色和微细胞毒性试验分析了该单克隆抗体的特异性反应性。SLE患者和其他胶原病患者中抗ATLA抗体的发生率没有差异。ES-1产生的单克隆抗体特异性地染色并杀死了HTLV1感染的细胞系，但不与其他人类淋巴细胞系反应。该单克隆抗体未能与SLE患者的外周血单个核细胞（PBMC）、丝裂原诱导的T细胞母细胞和碘脱氧尿苷处理的T细胞反应。因此，HTLV1在SLE病因学中的可能作用尚未确立。
