Elander A, Sjöström M, Lundgren F, Scherstén T, Bylund-Fellenius A C
Clin Sci (Lond). 1985 Aug;69(2):153-64. doi: 10.1042/cs0690153.
Two mitochondrial subpopulations were evaluated with biochemical and morphological techniques in human gastrocnemius muscle of 10 patients with peripheral arterial insufficiency and 12 control individuals. The subsarcolemmal mitochondria were released by gentle homogenization, with a recovery of 32-37%, and the intermyofibrillar by enzymic digestion and further mechanical disintegration, recovery 18-21%. The subsarcolemmal mitochondria were morphologically defined as those located within 2 micron from the sarcolemma membrane and the intermyofibrillar mitochondria as those located in the rest of the fibre. In the controls the intermyofibrillar mitochondria had a lower respiratory ratio than the subsarcolemmal, owing to a higher state II respiration. The subsarcolemmal space, which contained 25% of the mitochondria, had a mitochondrial volume density two- to three-fold that of the intermyofibrillar space in the controls. The patients, who had a 48-64% higher oxidative enzyme capacity in their muscle tissue, had higher respiratory rate and respiratory control index with similar ADP/O ratio in the subsarcolemmal fraction in comparison with the controls. The citrate synthase activity was higher in both mitochondrial fractions of the patients. The volume densities of mitochondria, total as well as for both subpopulations, were also higher in the patients, which was further reflected in higher yields of mitochondrial protein. The results demonstrate that both subpopulations of muscle mitochondria are able to adapt quantitatively and/or qualitatively. Furthermore, they show that the increased oxidative enzyme capacity of the patients is associated with an increased quantity of both mitochondrial populations and a qualitative improvement of the respiratory activity of the subsarcolemmal mitochondria.
运用生化和形态学技术，对10例外周动脉供血不足患者和12例对照个体的人腓肠肌中的两个线粒体亚群进行了评估。通过轻柔匀浆释放肌膜下线粒体，回收率为32%-37%，通过酶消化和进一步机械破碎释放肌原纤维间线粒体，回收率为18%-21%。肌膜下线粒体在形态上定义为位于距肌膜2微米范围内的线粒体，肌原纤维间线粒体定义为位于纤维其余部分的线粒体。在对照组中，由于状态II呼吸较高，肌原纤维间线粒体的呼吸比率低于肌膜下线粒体。含有25%线粒体的肌膜下间隙，其线粒体体积密度是对照组肌原纤维间间隙的两到三倍。患者肌肉组织中的氧化酶能力比对照组高48%-64%，与对照组相比，其肌膜下部分的呼吸速率和呼吸控制指数更高，ADP/O比率相似。患者的两个线粒体部分中的柠檬酸合酶活性均较高。患者线粒体的总体积密度以及两个亚群的体积密度也更高，这进一步体现在线粒体蛋白产量更高。结果表明，肌肉线粒体的两个亚群都能够在数量和/或质量上发生适应性变化。此外，结果还表明，患者氧化酶能力的增加与两个线粒体群体数量的增加以及肌膜下线粒体呼吸活性的质量改善有关。
