在卵巢储备功能减退的年轻女性的颗粒细胞中，参与细胞增殖的 microRNAs 的表达谱发生改变。
Micro-RNAs involved in cellular proliferation have altered expression profiles in granulosa of young women with diminished ovarian reserve.
机构信息
Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology, Keck School of Medicine, University of Southern California, 2020 Zonal Avenue, IRD 534, Los Angeles, CA, 90033, USA.
Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, KS, USA.
出版信息
J Assist Reprod Genet. 2018 Oct;35(10):1777-1786. doi: 10.1007/s10815-018-1239-9. Epub 2018 Jul 9.
PURPOSE
The study aims to determine differences in micro-RNA (miRNA) expression in granulosa (GC) and cumulus cells (CC) between young women with diminished ovarian reserve (DOR) or normal ovarian reserve (NOR). Secondary objective was to identify downstream signaling pathways that could ultimately indicate causes of lower developmental competence of oocytes from young women with DOR.
METHODS
The method of the study is prospective cohort study.
RESULTS
Of the miRNA, 125 are differentially expressed in GC between DOR and NOR. Only nine miRNA were different in CC; therefore, we focused analysis on GC. In DOR GC, miR-100-5p, miR-16-5p, miR-30a-3p, and miR-193a-3p were significantly downregulated, while miR-155-5p, miR-192-5p, miR-128-3p, miR-486-5p, miR130a-3p, miR-92a-3p, miR-17-3p, miR-221-3p, and miR-175p were increased. This pattern predicted higher cell proliferation in the DOR GC. The primary pathways include MAPK, Wnt, and TGFbeta.
CONCLUSIONS
The miRNA pattern identified critical functions in cell proliferation and survival associated with DOR. GC in women with DOR seems to respond differently to the LH surge.
目的
本研究旨在比较卵巢储备功能正常（NOR）和降低（DOR）的年轻女性的颗粒细胞（GC）和卵丘细胞（CC）中微小 RNA（miRNA）的表达差异。次要目的是确定下游信号通路，这些通路最终可能表明 DOR 年轻女性卵母细胞发育能力降低的原因。
方法
研究方法为前瞻性队列研究。
结果
在 DOR 和 NOR 的 GC 之间，有 125 种 miRNA 表达存在差异。在 CC 中仅有 9 种 miRNA 不同；因此，我们重点对 GC 进行了分析。在 DOR 的 GC 中，miR-100-5p、miR-16-5p、miR-30a-3p 和 miR-193a-3p 表达显著下调，而 miR-155-5p、miR-192-5p、miR-128-3p、miR-486-5p、miR130a-3p、miR-92a-3p、miR-17-3p、miR-221-3p 和 miR-175p 表达增加。这种模式预示着 DOR 的 GC 细胞增殖较高。主要通路包括 MAPK、Wnt 和 TGFbeta。
结论
所确定的 miRNA 模式对与 DOR 相关的细胞增殖和存活具有关键功能。DOR 女性的 GC 似乎对 LH 峰有不同的反应。
Zotero 插件