人类int-1乳腺癌基因的核苷酸序列；编码序列和非编码序列的进化保守性。
The nucleotide sequence of the human int-1 mammary oncogene; evolutionary conservation of coding and non-coding sequences.
作者信息
van Ooyen A, Kwee V, Nusse R
出版信息
EMBO J. 1985 Nov;4(11):2905-9. doi: 10.1002/j.1460-2075.1985.tb04021.x.
Abstract
The mouse mammary tumor virus can induce mammary tumors in mice by proviral activation of an evolutionarily conserved cellular oncogene called int-1. Here we present the nucleotide sequence of the human homologue of int-1, and compare it with the mouse gene. Like the mouse gene, the human homologue contains a reading frame of 370 amino acids, with only four substitutions. The amino acid changes are all in the hydrophobic leader domain of the int-1 encoded protein, and do not significantly alter its hydropathic index. The conservation between the mouse and the human int-1 genes is not restricted to exons; extensive parts of the introns are also homologous. Thus, int-1 ranks among the most conserved genes known, a property shared with other oncogenes.
摘要
小鼠乳腺肿瘤病毒可通过一种名为int-1的进化保守细胞癌基因的原病毒激活在小鼠体内诱发乳腺肿瘤。在此，我们呈现int-1人类同源物的核苷酸序列，并将其与小鼠基因进行比较。与小鼠基因一样，人类同源物包含一个由370个氨基酸组成的阅读框，仅有四处替换。氨基酸变化均位于int-1编码蛋白的疏水前导结构域，并未显著改变其亲水性指数。小鼠和人类int-1基因之间的保守性不仅限于外显子；内含子的大片段也具有同源性。因此，int-1位列已知最保守的基因之中，这一特性与其他癌基因相同。
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本文引用的文献
1
Organization and expression of eucaryotic split genes coding for proteins.编码蛋白质的真核生物断裂基因的组织与表达。
Annu Rev Biochem. 1981;50:349-83. doi: 10.1146/annurev.bi.50.070181.002025.
2
Incidence of double minutes, cytogenetic equivalents of gene amplification, in human carcinoma cells.人类癌细胞中双微体（基因扩增的细胞遗传学等效物）的发生率。
Int J Cancer. 1984 Sep 15;34(3):369-73. doi: 10.1002/ijc.2910340313.
3
Molecular cloning and chromosomal assignment of the human homolog of int-1, a mouse gene implicated in mammary tumorigenesis.与小鼠乳腺肿瘤发生相关的int-1基因的人类同源基因的分子克隆及染色体定位
Mol Cell Biol. 1984 Nov;4(11):2532-4. doi: 10.1128/mcb.4.11.2532-2534.1984.
4
tk Enzyme expression in differentiating muscle cells is regulated through an internal segment of the cellular tk gene.在分化的肌肉细胞中，胸苷激酶（tk）酶的表达是通过细胞胸苷激酶基因的一个内部片段来调控的。
Mol Cell Biol. 1984 Sep;4(9):1777-84. doi: 10.1128/mcb.4.9.1777-1784.1984.
5
Immunoglobulin gene transcription is activated by downstream sequence elements.免疫球蛋白基因转录由下游序列元件激活。
Cell. 1983 Jul;33(3):741-8. doi: 10.1016/0092-8674(83)90016-8.
6
A lymphocyte-specific cellular enhancer is located downstream of the joining region in immunoglobulin heavy chain genes.一种淋巴细胞特异性细胞增强子位于免疫球蛋白重链基因连接区的下游。
Cell. 1983 Jul;33(3):729-40. doi: 10.1016/0092-8674(83)90015-6.
7
A tissue-specific transcription enhancer element is located in the major intron of a rearranged immunoglobulin heavy chain gene.一种组织特异性转录增强子元件位于重排的免疫球蛋白重链基因的主要内含子中。
Cell. 1983 Jul;33(3):717-28. doi: 10.1016/0092-8674(83)90014-4.
8
The molecular genetics of cellular oncogenes.细胞癌基因的分子遗传学
Annu Rev Genet. 1984;18:553-612. doi: 10.1146/annurev.ge.18.120184.003005.
9
Cellular oncogenes and retroviruses.细胞癌基因与逆转录病毒。
Annu Rev Biochem. 1983;52:301-54. doi: 10.1146/annurev.bi.52.070183.001505.
10
Human epidermal growth factor receptor cDNA sequence and aberrant expression of the amplified gene in A431 epidermoid carcinoma cells.人表皮生长因子受体cDNA序列及扩增基因在A431表皮样癌细胞中的异常表达。
Nature. 1984;309(5967):418-25. doi: 10.1038/309418a0.
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