van Ooyen A, Kwee V, Nusse R
EMBO J. 1985 Nov;4(11):2905-9. doi: 10.1002/j.1460-2075.1985.tb04021.x.
The mouse mammary tumor virus can induce mammary tumors in mice by proviral activation of an evolutionarily conserved cellular oncogene called int-1. Here we present the nucleotide sequence of the human homologue of int-1, and compare it with the mouse gene. Like the mouse gene, the human homologue contains a reading frame of 370 amino acids, with only four substitutions. The amino acid changes are all in the hydrophobic leader domain of the int-1 encoded protein, and do not significantly alter its hydropathic index. The conservation between the mouse and the human int-1 genes is not restricted to exons; extensive parts of the introns are also homologous. Thus, int-1 ranks among the most conserved genes known, a property shared with other oncogenes.
小鼠乳腺肿瘤病毒可通过一种名为int-1的进化保守细胞癌基因的原病毒激活在小鼠体内诱发乳腺肿瘤。在此，我们呈现int-1人类同源物的核苷酸序列，并将其与小鼠基因进行比较。与小鼠基因一样，人类同源物包含一个由370个氨基酸组成的阅读框，仅有四处替换。氨基酸变化均位于int-1编码蛋白的疏水前导结构域，并未显著改变其亲水性指数。小鼠和人类int-1基因之间的保守性不仅限于外显子；内含子的大片段也具有同源性。因此，int-1位列已知最保守的基因之中，这一特性与其他癌基因相同。
