Moore R, Casey G, Brookes S, Dixon M, Peters G, Dickson C
EMBO J. 1986 May;5(5):919-24. doi: 10.1002/j.1460-2075.1986.tb04304.x.
A major proportion of carcinomas induced by mouse mammary tumour virus (MMTV) show evidence for proviral activation of a cellular gene, int-2, on chromosome 7. The sequence of 7869 bp of DNA spanning the transcription unit of int-2 was determined and compared with that of a series of int-2-specific cDNA clones derived from mammary tumour RNA. The predicted positions of intron-exon boundaries, established by alignment of cDNA and chromosomal DNA sequences, indicate that the gene comprises at least three exons. An open reading frame capable of encoding a protein of 245 amino acids with an estimated mol. wt of 27 kd, is flanked by substantial non-coding segments at both 5' and 3' ends. Comparison of the chromosomal DNA sequence and the predicted amino acid sequence with available data-bases has revealed no homology to other known genes. These results are discussed in relation to the status of int-2 as a candidate proto-oncogene.
由小鼠乳腺肿瘤病毒（MMTV）诱导产生的大部分癌瘤显示出7号染色体上细胞基因int-2原病毒激活的证据。测定了跨越int-2转录单位的7869bp DNA序列，并将其与一系列源自乳腺肿瘤RNA的int-2特异性cDNA克隆的序列进行比较。通过cDNA与染色体DNA序列比对确定的内含子-外显子边界的预测位置表明该基因至少包含三个外显子。一个能够编码245个氨基酸、估计分子量为27kd的蛋白质的开放阅读框，在5'和3'端两侧均有大量非编码片段。将染色体DNA序列和预测的氨基酸序列与现有数据库进行比较，未发现与其他已知基因有同源性。结合int-2作为候选原癌基因的状态对这些结果进行了讨论。
