Ponzi M, Pace T, Dore E, Frontali C
EMBO J. 1985 Nov;4(11):2991-5. doi: 10.1002/j.1460-2075.1985.tb04034.x.
A fragment of Plasmodium berghei DNA was cloned using a technique designed to select for telomeric sequences. The cloned fragment recognizes Bal31-sensitive bands in P. berghei genomic digests. It contains at its distal end at least 70 tandem repeats of the heptanucleotide sequence CCCTGAAA. The presence of natural single strand discontinuities in the telomeric regions of P. berghei DNA is demonstrated by the selective incorporation of deoxyribonucleoside triphosphates in the absence of DNase. The number of copies of the cloned sequence present in each genome agrees with an estimate of 6-12 chromosomes per nucleus.
使用一种旨在筛选端粒序列的技术克隆了伯氏疟原虫DNA的一个片段。该克隆片段在伯氏疟原虫基因组消化物中识别对Bal31敏感的条带。它在其远端包含至少70个七核苷酸序列CCCTGAAA的串联重复。在没有DNase的情况下，脱氧核糖核苷三磷酸的选择性掺入证明了伯氏疟原虫DNA端粒区域存在天然单链间断。每个基因组中存在的克隆序列的拷贝数与每个细胞核有6 - 12条染色体的估计值相符。
