Feed Science Institute, College of Animal Science, Zhejiang University, No. 866, Yuhangtang Road, Hangzhou, 310058, Zhejiang Province, China.
College of Animal Science and Technology, Zhejiang Agricultural and Forestry University, Lin'an, Hangzhou, 311300, Zhejiang Province, China.
Biol Trace Elem Res. 2019 Apr;188(2):478-484. doi: 10.1007/s12011-018-1430-y. Epub 2018 Jul 9.
This study was conducted to investigate the effects of different selenomethionine (SM) forms and levels on productive performance and antioxidant status of broiler breeders and its offspring. Four hundred eighty 48-week-old Lingnan Yellow broiler breeders were randomly divided into four groups, provided basal diet with 0.15 or 0.30 mg/kg Se coming from two SM forms of DL-SM and L-SM. The experiment included a 4-week pretreatment period and an 8-week trial period. During the trial period, eggs were incubated once a week under standard conditions. The broiler breeders were slaughtered after the trial period. At the same time, 15 1-day-old chicks were selected at random per replicate and killed. The results showed that different SM forms and levels had no significant differences in average egg weight, feed intake, and feed-to-egg ration. The DL-SM group in contrast to the L-SM group induced a notable elevation of glutathione peroxidase (GPx) activity in serum (P < 0.01) and liver (P < 0.05), and the 0.15 mg/kg group had higher GPx activity than 0.30 mg/kg in serum (P < 0.01) and pancreas (P < 0.05). Different SM forms showed no significant differences in total antioxidant capability (T-AOC). Diets with 0.15 mg/kg Se exhibited a higher level of T-AOC in serum (P < 0.01) and some tissues. Besides, malondialdehyde (MDA) concentrations in serum, liver, and kidney significantly decreased due to the supplementation of DL-SM. Supplemental 0.15 mg/kg Se reduced MDA concentrations in kidney and muscle. The offspring of broiler breeders fed on DL-SM had higher GPx activity in liver and kidney than L-SM treatment. Supplemental 0.15 mg/kg Se also improved GPx activity in kidney and muscle and T-AOC in kidney of 1-day-old chicks. In summary, our study demonstrated that compared with L-SM, DL-SM was more effective for enhancing the antioxidant status of broiler breeders and its offspring. Moreover, the recommended level of Se supplementation was 0.15 mg/kg Se in Lingnan Yellow broiler breeder diets.
本研究旨在探讨不同硒代蛋氨酸（SM）形式和水平对种鸡生产性能和抗氧化状态及其后代的影响。将 480 只 48 周龄岭南黄种鸡随机分为 4 组，分别提供基础日粮，其中硒来自两种 SM 形式的 DL-SM 和 L-SM，含量分别为 0.15 或 0.30mg/kg。试验包括 4 周的预处理期和 8 周的试验期。在试验期间，每周在标准条件下孵化一次鸡蛋。试验结束后，种鸡被屠宰。同时，每个重复随机选择 15 只 1 日龄雏鸡并将其处死。结果表明，不同 SM 形式和水平对平均蛋重、采食量和饲料蛋比无显著影响。与 L-SM 组相比，DL-SM 组显著提高了血清（P<0.01）和肝脏（P<0.05）中谷胱甘肽过氧化物酶（GPx）的活性，0.15mg/kg 组血清（P<0.01）和胰腺（P<0.05）中 GPx 的活性高于 0.30mg/kg。不同 SM 形式对总抗氧化能力（T-AOC）无显著影响。0.15mg/kg Se 组血清（P<0.01）和部分组织 T-AOC 水平较高。此外，由于添加了 DL-SM，血清、肝脏和肾脏中的丙二醛（MDA）浓度显著降低。补充 0.15mg/kg Se 降低了肾脏和肌肉中的 MDA 浓度。饲喂 DL-SM 的种鸡后代的肝脏和肾脏中的 GPx 活性高于 L-SM 处理。补充 0.15mg/kg Se 还提高了 1 日龄雏鸡肾脏和肌肉中的 GPx 活性以及肾脏中的 T-AOC。综上所述，本研究表明，与 L-SM 相比，DL-SM 更有效地增强了种鸡及其后代的抗氧化状态。此外，岭南黄种鸡日粮中硒的推荐添加量为 0.15mg/kg Se。
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