Kosmider Beata, Mason Robert J, Bahmed Karim
Department of Thoracic Medicine and Surgery, Temple University, Philadelphia, PA, USA.
Center for Inflammation, Translational and Clinical Lung Research, Temple University, Philadelphia, PA, USA.
Methods Mol Biol. 2018;1809:83-90. doi: 10.1007/978-1-4939-8570-8_7.
Alveolar type II (ATII) cells synthesize, store, and secrete pulmonary surfactant and restore the epithelium after damage to the alveolar epithelium. Isolation of human ATII cells provides a valuable tool to study their function under normal and pathophysiological conditions. Moreover, maintenance of their differentiated phenotype in vitro allows further study of their function. Here we describe a protocol for efficient ATII cell isolation, characterization, and culture.
肺泡II型（ATII）细胞合成、储存和分泌肺表面活性物质，并在肺泡上皮受损后修复上皮。分离人ATII细胞为研究其在正常和病理生理条件下的功能提供了一个有价值的工具。此外，在体外维持其分化表型有助于进一步研究其功能。在此，我们描述一种高效分离、鉴定和培养ATII细胞的方法。
