大鼠和小鼠肺泡Ⅱ型上皮细胞的分离
Isolation of Rat and Mouse Alveolar Type II Epithelial Cells.
作者信息
Jansing Nicole L, McClendon Jazalle, Kage Hidenori, Sunohara Mitsuhiro, Alvarez Juan R, Borok Zea, Zemans Rachel L
机构信息
Division of Pulmonary, Critical Care, and Sleep Medicine, Department of Medicine, National Jewish Health, Denver, CO, USA.
Division of Pulmonary, Critical Care and Sleep Medicine, Department of Medicine, Hastings Center for Pulmonary Research, Los Angeles, CA, USA.
出版信息
Methods Mol Biol. 2018;1809:69-82. doi: 10.1007/978-1-4939-8570-8_6.
Abstract
The gas exchange surface of the lungs is lined by an epithelium consisting of alveolar type (AT) I and ATII cells. ATII cells function to produce surfactant, play a role in host defense and fluid and ion transport, and serve as progenitors. ATI cells are important for gas exchange and fluid and ion transport. Our understanding of the biology of these cells depends on the investigation of isolated cells. Here, we present methods for the isolation of mouse and rat ATII cells.
摘要
肺的气体交换表面由一层上皮细胞构成，该上皮细胞由肺泡I型（AT）细胞和II型细胞组成。II型肺泡细胞的功能是产生表面活性剂，在宿主防御以及液体和离子运输中发挥作用，并作为祖细胞。I型肺泡细胞对气体交换以及液体和离子运输很重要。我们对这些细胞生物学特性的了解依赖于对分离细胞的研究。在此，我们介绍分离小鼠和大鼠II型肺泡细胞的方法。
相似文献
1
Isolation of Rat and Mouse Alveolar Type II Epithelial Cells.大鼠和小鼠肺泡Ⅱ型上皮细胞的分离
Methods Mol Biol. 2018;1809:69-82. doi: 10.1007/978-1-4939-8570-8_6.
2
Isolation and Characterization of Human Alveolar Type II Cells.人肺泡Ⅱ型细胞的分离与鉴定
Methods Mol Biol. 2018;1809:83-90. doi: 10.1007/978-1-4939-8570-8_7.
3
Isolation of Alveolar Type II Cells from Adult Bovine Lung.从成年牛肺中分离肺泡Ⅱ型细胞。
Curr Protoc Toxicol. 2019 Jun;80(1):e71. doi: 10.1002/cptx.71. Epub 2019 Mar 15.
4
Isolation of alveolar epithelial type II progenitor cells from adult human lungs.从成人肺中分离肺泡上皮 II 型祖细胞。
Lab Invest. 2011 Mar;91(3):363-78. doi: 10.1038/labinvest.2010.187. Epub 2010 Nov 15.
5
Mechanisms of ATII-to-ATI Cell Differentiation during Lung Regeneration.肺泡 II 型细胞到肺泡 I 型细胞分化在肺再生中的机制。
Int J Mol Sci. 2020 Apr 30;21(9):3188. doi: 10.3390/ijms21093188.
6
Enolase 1 (ENO1) and protein disulfide-isomerase associated 3 (PDIA3) regulate Wnt/β-catenin-driven trans-differentiation of murine alveolar epithelial cells.烯醇化酶1（ENO1）和蛋白二硫键异构酶相关蛋白3（PDIA3）调节Wnt/β-连环蛋白驱动的小鼠肺泡上皮细胞转分化。
Dis Model Mech. 2015 Aug 1;8(8):877-90. doi: 10.1242/dmm.019117. Epub 2015 May 14.
7
Isolation and characterisation of alveolar type II pneumocytes from adult bovine lung.从成年牛肺中分离和鉴定肺泡 II 型细胞。
Sci Rep. 2018 Aug 9;8(1):11927. doi: 10.1038/s41598-018-30234-x.
8
Mechanical stretch activates piezo1 in caveolae of alveolar type I cells to trigger ATP release and paracrine stimulation of surfactant secretion from alveolar type II cells.机械拉伸激活肺泡 I 型细胞小窝中的 Piezo1，触发 ATP 释放，并激活肺泡 II 型细胞的旁分泌表面活性物质分泌。
FASEB J. 2020 Sep;34(9):12785-12804. doi: 10.1096/fj.202000613RRR. Epub 2020 Aug 3.
9
Unbiased Quantitation of Alveolar Type II to Alveolar Type I Cell Transdifferentiation during Repair after Lung Injury in Mice.小鼠肺损伤修复过程中Ⅱ型肺泡上皮细胞向Ⅰ型肺泡上皮细胞转分化的无偏定量分析
Am J Respir Cell Mol Biol. 2017 Nov;57(5):519-526. doi: 10.1165/rcmb.2017-0037MA.
10
Defining signals that promote human alveolar type I differentiation.定义促进人肺泡 I 型细胞分化的信号。
Am J Physiol Lung Cell Mol Physiol. 2024 Apr 1;326(4):L409-L418. doi: 10.1152/ajplung.00191.2023. Epub 2024 Feb 13.
引用本文的文献
1
Endothelial CLEC5A drives barrier dysfunction and vascular leakage responsible for lung injury in bacterial pneumonia and sepsis.内皮细胞CLEC5A导致屏障功能障碍和血管渗漏，这是细菌性肺炎和脓毒症中肺损伤的原因。
Sci Adv. 2025 Jun 13;11(24):eadt7589. doi: 10.1126/sciadv.adt7589. Epub 2025 Jun 11.
2
MicroRNA-21-5p regulates autophagy and apoptosis via the activation of the PI3K/AKT/mTOR signaling pathway and reducing HO-induced damage in primary AEC-II.微小RNA-21-5p通过激活PI3K/AKT/mTOR信号通路和减轻高氧诱导的原代II型肺泡上皮细胞损伤来调节自噬和凋亡。
Int J Immunopathol Pharmacol. 2025 Jan-Dec;39:3946320251343369. doi: 10.1177/03946320251343369. Epub 2025 Jun 9.
3
Blocking IL-23 Signaling Mitigates Cigarette Smoke-Induced Murine Emphysema.阻断 IL-23 信号通路可减轻香烟烟雾诱导的小鼠肺气肿。
Environ Toxicol. 2024 Dec;39(12):5334-5346. doi: 10.1002/tox.24405. Epub 2024 Sep 2.
4
Adiponectin suppresses stiffness-dependent, profibrotic activation of lung fibroblasts.脂联素抑制依赖于硬度的肺成纤维细胞的促纤维化激活。
Am J Physiol Lung Cell Mol Physiol. 2024 Oct 1;327(4):L487-L502. doi: 10.1152/ajplung.00037.2024. Epub 2024 Aug 6.
5
Long-term alterations in lung epithelial cells after EL-RSV infection exacerbate allergic responses through IL-1β-induced pathways.EL-RSV 感染后肺上皮细胞的长期改变通过 IL-1β 诱导的途径加重过敏反应。
Mucosal Immunol. 2024 Oct;17(5):1072-1088. doi: 10.1016/j.mucimm.2024.07.007. Epub 2024 Jul 27.
6
Abnormal mitochondrial iron metabolism damages alveolar type II epithelial cells involved in bleomycin-induced pulmonary fibrosis.异常的线粒体铁代谢损害了参与博来霉素诱导的肺纤维化的肺泡 II 型上皮细胞。
Theranostics. 2024 Apr 15;14(7):2687-2705. doi: 10.7150/thno.94072. eCollection 2024.
7
Simultaneous isolation of proximal and distal lung progenitor cells from individual mice using a 3D printed guide reduces proximal cell contamination of distal lung epithelial cell isolations.使用 3D 打印引导器从单个小鼠中同时分离近端和远端肺祖细胞，可减少远端肺上皮细胞分离中近端细胞的污染。
Stem Cell Reports. 2022 Dec 13;17(12):2718-2731. doi: 10.1016/j.stemcr.2022.11.002. Epub 2022 Dec 1.
8
KIF2A decreases IL-33 production and attenuates allergic asthmatic inflammation.驱动蛋白家族成员2A（KIF2A）可减少白细胞介素-33（IL-33）的产生并减轻过敏性哮喘炎症。
Allergy Asthma Clin Immunol. 2022 Jun 19;18(1):55. doi: 10.1186/s13223-022-00697-9.
9
Lung Injury Induces Alveolar Type 2 Cell Hypertrophy and Polyploidy with Implications for Repair and Regeneration.肺损伤诱导肺泡 II 型细胞肥大和多倍体化，对修复和再生有影响。
Am J Respir Cell Mol Biol. 2022 May;66(5):564-576. doi: 10.1165/rcmb.2021-0356OC.
10
Bone marrow mesenchymal stem cell-conditioned medium facilitates fluid resolution via miR-214-activating epithelial sodium channels.骨髓间充质干细胞条件培养基通过激活上皮钠通道的miR-214促进液体消退。
MedComm (2020). 2020 Nov 12;1(3):376-385. doi: 10.1002/mco2.40. eCollection 2020 Dec.
本文引用的文献
1
Isolation and culture of alveolar epithelial Type I and Type II cells from rat lungs.从大鼠肺中分离和培养I型和II型肺泡上皮细胞。
Methods Mol Biol. 2013;945:145-59. doi: 10.1007/978-1-62703-125-7_10.
2
Efficient and rapid isolation and purification of mouse alveolar type II epithelial cells.高效快速分离和纯化小鼠肺泡II型上皮细胞。
Exp Lung Res. 2012 Sep;38(7):363-73. doi: 10.3109/01902148.2012.713077.
3
Characterization of mouse alveolar epithelial cell monolayers.小鼠肺泡上皮细胞单层的特性分析。
Am J Physiol Lung Cell Mol Physiol. 2009 Jun;296(6):L1051-8. doi: 10.1152/ajplung.00021.2009. Epub 2009 Mar 27.
4
Metabolic properties and ultrastructure of alveolar type II cells isolated with elastase.用弹性蛋白酶分离的Ⅱ型肺泡细胞的代谢特性和超微结构
Biochim Biophys Acta. 1980 Jun 23;618(3):510-23. doi: 10.1016/0005-2760(80)90270-2.
5
An improved method for isolating type II cells in high yield and purity.一种高产率、高纯度分离II型细胞的改进方法。
Am Rev Respir Dis. 1986 Jul;134(1):141-5. doi: 10.1164/arrd.1986.134.1.141.
6
Isolation and culture of alveolar type II cells.肺泡II型细胞的分离与培养。
Am J Physiol. 1990 Apr;258(4 Pt 1):L134-47. doi: 10.1152/ajplung.1990.258.4.L134.
7
Pulmonary alveolar type II cells isolated from rats. Release of phosphatidylcholine in response to beta-adrenergic stimulation.从大鼠分离出的肺泡II型细胞。对β-肾上腺素能刺激的反应中磷脂酰胆碱的释放。
J Clin Invest. 1979 Mar;63(3):378-87. doi: 10.1172/JCI109313.
Zotero 插件