Price Dominique N, Muttil Pavan
Department of Pharmaceutical Sciences, College of Pharmacy, University of New Mexico, Albuquerque, NM, USA.
Methods Mol Biol. 2018;1809:415-429. doi: 10.1007/978-1-4939-8570-8_27.
Pulmonary delivery in animal models can be performed using either direct administration methods or by passive inhalation. Direct pulmonary delivery requires the animal to be endotracheally intubated, whereas passive delivery uses a nose-only or a whole-body chamber. Endotracheal delivery of therapeutics and vaccines allows investigators to deliver the payload directly into the lung without the limitations associated with passive pulmonary administration methods. Additionally, endotracheal delivery can achieve deep lung delivery without the involvement of other exposure routes and is more reproducible and quantitative than passive pulmonary delivery in terms of accurate dosing. Here we describe the endotracheal delivery of both liquids and dry powders for preclinical models of treatment and exposure.
在动物模型中，肺部给药可以通过直接给药方法或被动吸入来进行。直接肺部给药要求对动物进行气管插管，而被动给药则使用仅针对鼻子或全身的吸入舱。通过气管内给药方式递送治疗药物和疫苗，使研究人员能够将有效载荷直接递送至肺部，而不受被动肺部给药方法相关的限制。此外，气管内给药可以在不涉及其他暴露途径的情况下实现肺部深部给药，并且在精确给药方面比被动肺部给药更具可重复性和定量性。在此，我们描述了用于治疗和暴露的临床前模型的液体和干粉的气管内给药方法。
