School of Life Science and Biotechnology , Yangtze Normal University , Chongqing 408100 , China.
School of Clinical Medicine , Jining Medical University , Jining 272067 , China.
J Agric Food Chem. 2018 Aug 1;66(30):8015-8025. doi: 10.1021/acs.jafc.8b02979. Epub 2018 Jul 20.
Consumption of pectin contributes to changes in the gut microbiota and the metabolism of short-chain fatty acids (SCFAs). We aimed to investigate the effects of and mechanism by which pectin prevented nonalcoholic fatty liver disease (NAFLD) in mice that were fed a high-fat diet containing 30% lard (HF). HF-fed mice that orally ingested pectin for 8 weeks exhibited improvements in lipid metabolism and decreased oxidative stress and inflammation through a mechanism regulated by the mitogen-activated protein kinase pathway. Pectin dose-dependently generated an increase in acetic acid (from 566.4 ± 26.6 to 694.6 ± 35.9 μmol/mL, p < 0.05) and propionic acid (from 474.1 ± 84.3 to 887.0 ± 184.7 μmol/mL, p < 0.05) contents and significantly increased the relative abundance of Bacteroides (from 0.27% to 11.6%), Parabacteroides (from 3.9‰ to 5.3%), Olsenella (from 2.9‰ to 1.3%), and Bifidobacterium (from 0.03% to 1.9%) in the gut of HF-fed mice. Intestinal microbiota and SCFAs may thus contribute to the well-established link between pectin consumption and NAFLD.
果胶的摄入会导致肠道微生物群和短链脂肪酸 (SCFA) 的代谢发生变化。我们旨在研究果胶如何通过丝裂原活化蛋白激酶途径调节的机制来预防高脂肪饮食（含 30%猪油）喂养的小鼠发生非酒精性脂肪性肝病（NAFLD）。果胶经口喂食 HF 喂养的小鼠 8 周后，可改善脂代谢，减少氧化应激和炎症。果胶呈剂量依赖性地增加乙酸（从 566.4 ± 26.6 增加到 694.6 ± 35.9 μmol/mL，p < 0.05）和丙酸（从 474.1 ± 84.3 增加到 887.0 ± 184.7 μmol/mL，p < 0.05）含量，并显著增加了肠道中拟杆菌（从 0.27%增加到 11.6%）、副拟杆菌（从 3.9‰增加到 5.3%）、奥尔森氏菌（从 2.9‰增加到 1.3%）和双歧杆菌（从 0.03%增加到 1.9%）的相对丰度。肠道微生物群和 SCFA 可能有助于果胶摄入与 NAFLD 之间的既定联系。
