J Agric Food Chem. 2019 Oct 30;67(43):11922-11930. doi: 10.1021/acs.jafc.9b03731. Epub 2019 Oct 16.
We investigated the regulatory effects of citrus pectin oligosaccharides (POS) from an innovative, chemically controllable degradation process on cholesterol metabolism and the gut microbial composition. The modulatory role of the intestinal flora was explored. Four-week-old male C57BL/6 mice were fed either a standard diet; a high-fat (HF) diet; or a HF diet with 0.15, 0.45, and 0.9 g/kg body weight POS for 30 days. POS reduced serum total cholesterol (TC) and low-density lipoprotein-cholesterol (LDL-C) in a dose-dependent manner. The relative abundances of specific bacterial groups in the feces and the concentrations of their metabolites were higher in the POS groups. There were significant correlations among , , and and short-chain fatty acids, as well as among serum TC, LDL-C, fecal bile acids, and liver cholesterol 7-α-hydroxylase and 3-hydroxy-3-methylglutaryl-coenzyme A reductase. These findings indicate that the prepared POS exhibited hypocholesterolemic effects and that the potential regulatory mechanisms of citrus POS on cholesterol metabolism are modulated by specific bacterial groups together with their metabolites.
我们研究了一种创新的、化学可控降解工艺得到的柑橘果胶低聚糖（POS）对胆固醇代谢和肠道微生物组成的调节作用。探索了肠道菌群的调节作用。4 周龄雄性 C57BL/6 小鼠分别用标准饮食、高脂肪（HF）饮食或含 0.15、0.45 和 0.9 g/kg 体重 POS 的 HF 饮食喂养 30 天。POS 呈剂量依赖性降低血清总胆固醇（TC）和低密度脂蛋白胆固醇（LDL-C）。粪便中特定细菌群的相对丰度和其代谢物的浓度在 POS 组中较高。 、 、 和短链脂肪酸之间以及血清 TC、LDL-C、粪便胆汁酸和肝脏胆固醇 7-α-羟化酶和 3-羟基-3-甲基戊二酰辅酶 A 还原酶之间存在显著相关性。这些发现表明，所制备的 POS 表现出降胆固醇作用，柑橘 POS 对胆固醇代谢的潜在调节机制是通过特定细菌群及其代谢物共同调节的。
