Department of Applied Physics and Science for Life Laboratory, KTH Royal Institute of Technology, Stockholm, Sweden.
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russian Federation.
Nat Methods. 2018 Aug;15(8):601-604. doi: 10.1038/s41592-018-0052-9. Epub 2018 Jul 9.
Reversibly photoswitchable fluorescent proteins (rsFPs) are gaining popularity as tags for optical nanoscopy because they make it possible to image with lower light doses. However, green rsFPs need violet-blue light for photoswitching, which is potentially phototoxic and highly scattering. We developed new rsFPs based on FusionRed that are reversibly photoswitchable with green-orange light. The rsFusionReds are bright and exhibit rapid photoswitching, thereby enabling nanoscale imaging of living cells.
可光激活荧光蛋白（rsFPs）作为光学纳米显微镜的标记物越来越受欢迎，因为它们可以用更低的光剂量进行成像。然而，绿色 rsFPs 需要使用蓝紫光进行光开关切换，这可能具有光毒性和高度散射性。我们基于 FusionRed 开发了新的 rsFPs，它们可以用绿-橙光进行可逆光开关切换。rsFusionReds 具有明亮的荧光和快速的光开关切换性能，从而能够对活细胞进行纳米级成像。
