Kim S H, Rinamalo M, Rainima-Qaniuci M, Talemaitoga N, Kama M, Rafai E, Choi M-H, Hong S T, Verweij J J, Kelly-Hope L, Stothard J R
Department of Parasitology, Liverpool School of Tropical Medicine, Liverpool, UK.
Ministry of Health, Dinem House, Suva, Republic of Fiji.
Parasite Epidemiol Control. 2016 Sep 5;1(3):263-267. doi: 10.1016/j.parepi.2016.08.005. eCollection 2016 Sep.
As a part of the lymphatic filariasis (LF) transmission assessment survey (TAS)/soil-transmitted helminths (STH) prevalence survey in Western Division of Fiji, a pilot screen for (SS) in school children was undertaken using a combination of the Baermann concentration (BC) method and real-time PCR assays. Using BC, faecal samples collected from 111 children of 7 schools were examined. A single child was positive for larvae of SS and underwent a clinical examination finding an asymptomatic infection. Other members of this child's household were screened with BC, finding none infected. Aliquots of 173 faecal samples preserved in ethanol originating from all schools were examined by real-time PCR, and the prevalence of SS infection was 3.5%. Our study confirms the existence of SS infection on Fiji and showed that assessing SS prevalence alongside TAS/STH survey is a convenient access platform, allowing introduction of other surveillance techniques such as BC and real-time PCR.
作为斐济西部地区淋巴丝虫病(LF)传播评估调查(TAS)/土壤传播蠕虫(STH)流行率调查的一部分,采用贝尔曼浓缩法(BC)和实时PCR检测相结合的方式,对在校儿童进行了类圆线虫(SS)的初步筛查。使用BC法对从7所学校收集的111名儿童的粪便样本进行了检测。一名儿童的SS幼虫呈阳性,并接受了临床检查,发现为无症状感染。该儿童家庭的其他成员经BC法筛查,未发现感染。对来自所有学校保存在乙醇中的173份粪便样本进行实时PCR检测,SS感染率为3.5%。我们的研究证实了斐济存在SS感染,并表明在TAS/STH调查中同时评估SS流行率是一个便捷的接入平台,可引入其他监测技术,如BC法和实时PCR。