Bersimbaev R I, Tairov M M, Salganik R I
Eur J Pharmacol. 1985 Sep 24;115(2-3):259-66. doi: 10.1016/0014-2999(85)90698-3.
The effects of prostaglandin E2 (PGE2) and inhibitors of RNA and protein synthesis on rat gastric mucosa were investigated in order to study the cellular and biochemical mechanisms involved in the PGE2-stimulated formation and secretion of gastric mucus. It was shown that PGE2 caused significant stimulation of gastric mucus secretion and this effect of PGE2 was inhibited by cycloheximide but not actinomycin D. The influence of PGE2 on the in vitro incorporation of N-acetyl-[3H]glucosamine and 14C-labelled amino acids in to the glycoproteins representing a major mucus component of the isolated gastric mucosa cells was also studied. The stimulatory effect of PGE2 on incorporation of labelled precursors into glycoproteins of gastric cells was also inhibited by cycloheximide. These results suggest that the effect of PGE2 on mucus production requires ongoing protein synthesis. cAMP can fully reproduce the effect of PGE2 on the formation and the secretion of gastric mucus. The binding of [3H]PGE2 to rat gastric non-parietal cell fractions consisting predominantly of mucoid cells correlated with the ability of PGE2 to increase adenylate cyclase activity in these cells. PGE2 had no effect on adenylate cyclase activity in cell suspensions enriched in parietal cells. These data suggest further that the stimulatory effect of PGE2 on mucus secretion may be mediated by cAMP as a messenger.
为了研究参与前列腺素E2（PGE2）刺激胃黏液形成和分泌的细胞及生化机制，对大鼠胃黏膜中PGE2以及RNA和蛋白质合成抑制剂的作用进行了研究。结果表明，PGE2可显著刺激胃黏液分泌，且PGE2的这一作用被放线菌酮抑制，但未被放线菌素D抑制。还研究了PGE2对体外将N-乙酰-[3H]葡糖胺和14C标记氨基酸掺入代表分离的胃黏膜细胞主要黏液成分的糖蛋白中的影响。放线菌酮也抑制了PGE2对标记前体掺入胃细胞糖蛋白的刺激作用。这些结果表明，PGE2对黏液产生的作用需要持续的蛋白质合成。环磷酸腺苷（cAMP）可完全重现PGE2对胃黏液形成和分泌的作用。[3H]PGE2与主要由黏液细胞组成的大鼠胃非壁细胞组分的结合，与PGE2增加这些细胞中腺苷酸环化酶活性的能力相关。PGE2对富含壁细胞的细胞悬液中的腺苷酸环化酶活性没有影响。这些数据进一步表明，PGE2对黏液分泌的刺激作用可能由cAMP作为信使介导。
