Pisarev M A, Hepburn A, Dumont J E
Experientia. 1985 Nov 15;41(11):1453-5. doi: 10.1007/BF01950031.
Treatment of dog thyroid slices with thyrotropin (TSH) results in an increase in ADP-ribosylation in nuclei isolated thereafter. This increase is time-dependent and is observed with concentrations of TSH eliciting physiological responses. The technique described here does not involve permeabilization of cell membranes, thereby avoiding artefacts which could arise from hypotonic shock. Cyclic AMP mimicked the stimulatory action of TSH.
用促甲状腺激素（TSH）处理犬甲状腺切片后，分离出的细胞核中ADP-核糖基化增加。这种增加呈时间依赖性，且在引发生理反应的TSH浓度下即可观察到。此处描述的技术不涉及细胞膜通透化，从而避免了因低渗休克可能产生的假象。环磷酸腺苷（cAMP）模拟了TSH的刺激作用。
