不同组织中的ADP-核糖基化蛋白共轭亚类。甲状腺激素对肝脏共轭物的特定影响。
ADP-ribosyl-protein conjugate subclasses in various tissues. Specific influence of thyroid hormone on liver conjugates.
作者信息
Lindner C, Hilz H
出版信息
Biochem J. 1982 Jul 15;206(1):61-5. doi: 10.1042/bj2060061.
The amounts of endogenous mono (ADP ribose)-protein conjugates and their hydroxyl-amine-sensitive and hydroxylamine-resistant subfractions in various tissues of the mouse were determined and compared with poly (ADP-ribose) conjugates. Total mono-(ADP-ribose) conjugates did not correlate with poly (ADP-ribose) residues or the cellularity of a tissue, but were related to the protein content and the amounts of NAD+ +NADH. The hydroxylamine-resistant subfraction, which in liver is mainly associated with the mitochondria [Adamietz, Wielckens, Bredehorst, Lengyel & Hilz (1981) Biochem. Biophys. Res, Commun. 101, 96-103], did not correlate with the tissue content of cytochrome c oxidase. In hypothyroid mice hydroxylamine-resistant mono (ADP-ribose) conjugates of the liver were increased by a factor of two while the hydroxylamine-sensitive conjugates did not change significantly under these conditions. Upon administration of thyroxine the hydroxylamine-resistant subfraction returned to normal.
测定了小鼠各种组织中内源性单（ADP核糖）-蛋白质缀合物及其对羟胺敏感和对羟胺抗性的亚组分的含量，并与聚（ADP核糖）缀合物进行了比较。总单（ADP核糖）缀合物与聚（ADP核糖）残基或组织的细胞数量无关，但与蛋白质含量以及NAD⁺+NADH的量有关。对羟胺抗性的亚组分在肝脏中主要与线粒体相关[Adamietz，Wielckens，Bredehorst，Lengyel和Hilz（1981年）《生物化学与生物物理学研究通讯》101，96 - 103]，与细胞色素c氧化酶的组织含量无关。在甲状腺功能减退的小鼠中，肝脏中对羟胺抗性的单（ADP核糖）缀合物增加了两倍，而在这些条件下对羟胺敏感的缀合物没有显著变化。给予甲状腺素后，对羟胺抗性的亚组分恢复正常。
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