Department of Biomedical Engineering, College of Engineering and Applied Sciences , Nanjing University , Nanjing , Jiangsu 210093 , P. R. China.
ACS Appl Mater Interfaces. 2018 Jul 25;10(29):24344-24348. doi: 10.1021/acsami.8b09222. Epub 2018 Jul 12.
As a milestone in DNA self-assembly, DNA origami has demonstrated powerful applications in many fields. However, the scarce availability of long single-stranded DNA (ssDNA) limits the size and sequences of DNA origami nanostructures, which in turn impedes the further development. In this study, we present a robust strategy to produce long circular ssDNA scaffold strands with custom-tailored lengths and sequences. These ssDNA products were then used as scaffolds for constructing various DNA origami nanostructures. This scalable method produces ssDNA at low cost with high purity and high yield, which can enable production of custom-designed DNA origami for various applications.
作为 DNA 自组装的一个里程碑，DNA 折纸术在许多领域展示了强大的应用。然而，长单链 DNA（ssDNA）的稀缺性限制了 DNA 折纸纳米结构的大小和序列，这反过来又阻碍了其进一步发展。在这项研究中，我们提出了一种强大的策略，用于生产具有定制长度和序列的长圆形 ssDNA 支架链。然后，这些 ssDNA 产物被用作构建各种 DNA 折纸纳米结构的支架。这种可扩展的方法以低成本、高纯度和高产率生产 ssDNA，这可以实现各种应用的定制 DNA 折纸术的生产。
