Sekiya S, Oosaki T, Suzuki N, Takamizawa H
Gynecol Oncol. 1985 Nov;22(3):324-33. doi: 10.1016/0090-8258(85)90047-2.
The chick chorioallantoic membrane (CAM) was used as an assay system to examine the invasive potential of human choriocarcinoma cell lines. When 5 X 10(6) cells were inoculated into the CAM at the 10th day of postfertilization, three of eight cell lines formed extensively invasive tumors within the CAM. A tendency to correlation between the tumorigenic potential of cell lines in hamster cheek pouches and their invasive potential in the CAM was noted. In addition, the invasive capacity of cell lines correlated well with the amount of collagenase but did not correlate with the amount of plasminogen activator or cathepsin B secreted by them. It is concluded that a heterogeneity of invasive potential in the CAM exists among human choriocarcinoma cell lines and the role of the collagenase secreted by them is suggested.
鸡胚绒毛尿囊膜（CAM）被用作一种检测系统，以检查人绒毛膜癌细胞系的侵袭潜力。在受精后第10天将5×10⁶个细胞接种到CAM上时，8个细胞系中有3个在CAM内形成了广泛侵袭性肿瘤。注意到细胞系在仓鼠颊囊中的致瘤潜力与其在CAM中的侵袭潜力之间存在相关性趋势。此外，细胞系的侵袭能力与胶原酶的量密切相关，但与它们分泌的纤溶酶原激活物或组织蛋白酶B的量无关。得出结论，人绒毛膜癌细胞系在CAM中的侵袭潜力存在异质性，并提示了它们分泌的胶原酶的作用。
