Wang S S, Cai R F, Chen J, Li R J, Jiang R S
Intervirology. 1985;24(3):140-6. doi: 10.1159/000149633.
Studies are reported on two outbreaks of epidemic diarrhea in China involving 19,007 patients. The first outbreak occurred in the northern part of the Guangxi Autonomous Region and extended south from April through September 1983. In June 1984 a second outbreak of 6,570 cases occurred in Guanyang, a county in the northern part of Guangxi. 125 fecal samples from the two outbreaks were cultured for bacteria; all of the samples except one were negative. Rotavirus particles were detected in 44.1% (15/34) of stool specimens examined by immune electron microscopy. The two outbreaks appeared to be caused by non-group-A rotaviruses, based on the pattern of electrophoretic migration of the RNA genome segments in polyacrylamide gels and on the lack of a detectable common group antigen(s) when tested by an enzyme-linked immunosorbent assay for group A rotaviruses. Complement-fixation tests also indicated that 37.5% of convalescent-phase sera from patients produced antibody to the isolated rotavirus. The total molecular weight of the RNA segments in the new Guangxi strain was calculated to be 10.69 X 10(6), with the 11 genome segments having molecular weights (X 10(6)) of 2.19, 1.83, 1.56, 1.53, 0.82, 0.81, 0.60, 0.46, 0.35, 0.30, and 0.24. This study also reports comparative detection rates for direct and immune electron microscopy and for PAGE. PAGE proved to be the most sensitive detection method for these new rotaviruses.
据报道，中国发生了两起流行性腹泻疫情，涉及19007名患者。第一起疫情发生在广西自治区北部，于1983年4月至9月向南蔓延。1984年6月，广西北部的灌阳县发生了第二起疫情，有6570例病例。对两起疫情中的125份粪便样本进行了细菌培养；除一份样本外，所有样本均为阴性。通过免疫电子显微镜检查，在44.1%（15/34）的粪便标本中检测到轮状病毒颗粒。根据RNA基因组片段在聚丙烯酰胺凝胶中的电泳迁移模式，以及用A组轮状病毒酶联免疫吸附试验检测时缺乏可检测到的共同组抗原，这两起疫情似乎是由非A组轮状病毒引起的。补体结合试验还表明，37.5%的患者恢复期血清产生了针对分离出的轮状病毒的抗体。计算出新的广西毒株RNA片段的总分子量为10.69×10⁶，11个基因组片段的分子量（×10⁶）分别为2.19、1.83、1.56、1.53、0.82、0.81、0.60、0.46、0.35、0.30和0.24。本研究还报告了直接电子显微镜和免疫电子显微镜以及聚丙烯酰胺凝胶电泳（PAGE）的比较检测率。PAGE被证明是检测这些新型轮状病毒最敏感的方法。
