Research Center Oltchim, RamnicuValcea, Romania.
National Institute of Research and Development for Biological Sciences, Bucharest, Romania.
PLoS One. 2018 Jul 10;13(7):e0198121. doi: 10.1371/journal.pone.0198121. eCollection 2018.
Stable Schiff bases containing a furoxan moiety are synthesized as single regioisomers by the reaction of 3-methyl-2-oxy-furazan-4-carbaldehydewith various amino compounds at room temperature. The structures of synthesized compounds were fully characterized by multinuclear NMR spectroscopy and X-ray crystallography. The effect of synthesized Schiff bases containing a furoxan moiety on biological generation of reactive oxygen species and nitric oxide in plant tissues was investigated for the first time by fluorescence microscopy and the released NO identified as nitrite with Griess reagent. There is a good correlation between the biological generation of NO determined by fluorescence microscopy and with Griess reagent. Some of the synthesized compounds exhibited both nitric oxide and reactive oxygen species generation abilities and represent potential NO donors in plant tissues.
稳定的席夫碱含有呋咱部分，通过 3-甲基-2-氧代-呋喃-4-甲醛与各种氨基化合物在室温下反应合成，为单一区域异构体。通过多核 NMR 光谱和 X 射线晶体学对合成化合物的结构进行了充分的表征。首次通过荧光显微镜研究了含呋咱席夫碱对植物组织中活性氧和一氧化氮生物生成的影响，并使用格里斯试剂将释放的 NO 鉴定为亚硝酸盐。荧光显微镜法和格里斯试剂法测定的 NO 生物生成之间存在良好的相关性。一些合成化合物表现出同时产生一氧化氮和活性氧的能力，是植物组织中潜在的一氧化氮供体。
