Department of Industrial Engineering, University of Salerno, via Giovanni Paolo II, 132 - 84084 Fisciano, SA, Italy; NANO_MATES Research Centre, University of Salerno, via Giovanni Paolo II, 132 - 84084 Fisciano, SA, Italy.
Department of Industrial Engineering, University of Salerno, via Giovanni Paolo II, 132 - 84084 Fisciano, SA, Italy.
Bioresour Technol. 2018 Oct;266:431-438. doi: 10.1016/j.biortech.2018.06.108. Epub 2018 Jul 2.
Physical adsorption preserving activity and support reusability was used to directly bond lipase from Thermomyces lanuginosus on citric acid (CA) modified FeO/Au magnetic nanoparticles. A new faster approach has been used for CA ligand exchange, which ensures an high payload of stable enzyme. The immobilized lipase was tested for the biodiesel production from spent coffee ground in a solvent free system. It retains, after 60 days, more than 90% of its initial activity. Biodiesel yield of 51.7%, after 3 h of synthesis, which increases up to ∼100% after 24 h indicating an enzymatic fast kinetic, was measured. No significant decrease, during the first three cycles of use, of the lipase activity occurs. The biodiesel presents an ester content of 98.4 ± 0.23 in agreement with the EN14214. The iodine value of 76.67 (g iodine/100 g) is in agreement with the European standard.
物理吸附保持活性并支持重复使用，用于将来自Thermomyces lanuginosus 的脂肪酶直接键合到柠檬酸（CA）修饰的 FeO/Au 磁性纳米颗粒上。已经使用了一种新的更快的 CA 配体交换方法，可确保稳定酶的高载量。在无溶剂体系中，固定化脂肪酶用于从废咖啡渣生产生物柴油。在 60 天后，它保留了初始活性的 90%以上。在 3 小时的合成后，测量到 51.7%的生物柴油产率，在 24 小时后增加到约 100%，表明酶具有快速的动力学。在使用的前三个循环中，脂肪酶活性没有明显下降。生物柴油的酯含量为 98.4±0.23，符合 EN14214 标准。碘值为 76.67（g 碘/100g），符合欧洲标准。
