Alonazi Mona, Al-Diahan Sooad K, Alzahrani Zaenab R A, Ben Bacha Abir
Biochemistry Department, College of Sciences, King Saud University, P.O Box 22455, Riyadh, Saudi Arabia.
Saudi J Biol Sci. 2023 Sep;30(9):103772. doi: 10.1016/j.sjbs.2023.103772. Epub 2023 Aug 10.
This work describes the enzymatic transesterification of the oil extracted from SCGs for synthesis of biodiesel as a promising alternative to diesel fuels based on petroleum. Biocatalysts from various sources were tested for biodiesel synthesis using coffee oil among which CaCO- immobilized and showed the highest conversion yields (61 ± 2.64% and 64.3 ± 1.53%, respectively) in 4 h. In further optimizing reaction parameters, methanol to oil molar ratio, biocatalyst quantity, water content, as well as incubation time and temperature markedly improved oil-to-biodiesel conversion up to 99.33 ± 0.57 % in a solvent free reaction after 12 h at 55 °C. A mixture of inexpensive CaCO-immobilized bacterial lipases at a 1:1 ratio was the best environment-friendly catalyst for biofuel synthesis as well as the ideal trade-off between conversion and cost. Obtained coffee biodiesel remained stable beyond 40 days at ambient storage conditions and its chemical characteristics were comparable to those of other known biodiesels according to the European requirements (EN14214). Collectively, SCGs, after oil extraction, could be an ideal substrate for the production of an environment-friendly biodiesel by using appropriate mixture of CaCO-immobilized lipases.
这项工作描述了从虾壳废弃物中提取的油脂进行酶促酯交换反应以合成生物柴油，作为基于石油的柴油燃料的一种有前景的替代品。使用咖啡油对来自各种来源的生物催化剂进行了生物柴油合成测试，其中碳酸钙固定化的[具体生物催化剂名称未明确]在4小时内显示出最高的转化率（分别为61±2.64%和64.3±1.53%）。在进一步优化反应参数时，甲醇与油的摩尔比、生物催化剂用量、含水量以及孵育时间和温度在55℃下无溶剂反应12小时后，显著提高了油到生物柴油的转化率，最高可达99.33±0.57%。以1:1比例混合的廉价碳酸钙固定化细菌脂肪酶是生物燃料合成的最佳环保催化剂，也是转化率和成本之间的理想权衡。根据欧洲要求（EN14214），获得的咖啡生物柴油在常温储存条件下40多天内保持稳定，其化学特性与其他已知生物柴油相当。总体而言，提取油脂后的虾壳废弃物通过使用适当混合的碳酸钙固定化脂肪酶，可能成为生产环保生物柴油的理想底物。
