Zhang Bo, Yan Zunqiang, Gao Yi, Li Jiyou, Wang Zike, Wang Pengfei, Yang Qiaoli, Huang Xiaoyu, Gun Shuangbao
College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, China.
Jilin Rongtai Agricultural Development Co., Ltd., Changchun, China.
Front Vet Sci. 2022 Oct 18;9:942669. doi: 10.3389/fvets.2022.942669. eCollection 2022.
Precocious puberty is closely related to testicular development and spermatogenesis, and there is increasing evidence that miRNAs are involved in regulation of testicular development and spermatogenesis. However, little is known about the regulation of microRNAs (miRNAs) during precocious maturation in Hezuo (HZ) boars. In this study, serum Testosterone (T), Estradiol (E), Follicle-stimulating hormone (FSH) and Luteinizing hormone (LH) levels were detected in HZ and Landrace (LC) boars in the postnatal period at 30, 90, 120, 180, and 240 days, and the testes of HZ and LC boars at 30 and 120 days were used for histological observation. In addition, we performed small RNA-Seq to identify miRNA at sexual immaturity (30-days-old) and maturity (120-days-old) of HZ boar testis (using LC boar as control) to reveal the key miRNA in regulation of precocious puberty. Hormone assay results showed that high levels of T, E, FSH, and LH may be related to precocious sexual maturity of HZ boars, and that FSH may play an important function before sexual maturity. Histological observation showed that HZ boars developed earlier than LC boars and had reached sexual maturity at 120 days. Small RNA-Seq yielded a total of 359 exist miRNAs, 767 known miRNAs and 322 novel miRNAs in 12 samples; 549, 468, 133, and 247 differentially expressed (DE) miRNAs were identified between Ha vs. Hb, La vs. Lb, Ha vs. La, and Hb vs. Lb (log fold change >1 and < 0.05). Enrichment analysis showed that target genes of these DE miRNAs were enriched in many gene ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) signaling pathways (such as PI3K-Akt, Hippo and Rap1 signaling pathways) were related to testicular development and spermatogenesis. Further screening, some miRNAs (such as ssc-miR-29b, ssc-miR-199b, ssc-miR-383, ssc-miR-149, ssc-miR-615, and ssc-miR-370) were possibly associated with precocious puberty. These results provide new light on miRNA regulatory mechanisms involved in precocious puberty.
性早熟与睾丸发育和精子发生密切相关，越来越多的证据表明，微小RNA（miRNA）参与睾丸发育和精子发生的调控。然而，关于合作（HZ）公猪早熟过程中微小RNA（miRNA）的调控知之甚少。在本研究中，检测了HZ和长白（LC）公猪出生后30、90、120、180和240天血清睾酮（T）、雌二醇（E）、促卵泡激素（FSH）和促黄体生成素（LH）水平，并对30和120天的HZ和LC公猪睾丸进行组织学观察。此外，我们进行了小RNA测序，以鉴定HZ公猪睾丸性未成熟（30日龄）和成熟（120日龄）时的miRNA（以LC公猪作为对照），以揭示性早熟调控中的关键miRNA。激素检测结果表明，高水平的T、E、FSH和LH可能与HZ公猪性早熟有关，且FSH可能在性成熟前发挥重要作用。组织学观察表明，HZ公猪比LC公猪发育更早，在120天达到性成熟。小RNA测序在12个样本中共获得359个已知miRNA、767个已知miRNA和322个新miRNA；在Ha与Hb、La与Lb、Ha与La以及Hb与Lb之间分别鉴定出549、468,133和247个差异表达（DE）miRNA（对数倍变化>1且<0.05）。富集分析表明，这些DE miRNA的靶基因在许多基因本体（GO）术语中富集，京都基因与基因组百科全书（KEGG）信号通路（如PI3K-Akt、Hippo和Rap1信号通路）与睾丸发育和精子发生有关。进一步筛选发现，一些miRNA（如ssc-miR-29b、ssc-miR-199b、ssc-miR-383、ssc-miR-149、ssc-miR-615和ssc-miR-370）可能与性早熟有关。这些结果为性早熟中miRNA调控机制提供了新的线索。
