Meller K A, Całka J, Kaczmarek M, Jana B
Division of Reproductive Biology, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Tuwima 10 Str., 10-748 Olsztyn, Poland.
Division of Clinical Physiology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego 14 Str., 11-041 Olsztyn, Poland.
Theriogenology. 2018 Oct 1;119:96-104. doi: 10.1016/j.theriogenology.2018.06.025. Epub 2018 Jun 28.
Under physiological conditions, noradrenaline (NA) and adrenergic receptors (ARs) are implicated in the function of the uterus. The role of NA and the expression of ARs in the inflamed uterus is not fully understood. The aim of the present study was to determine the effect of inflammation on the levels of α1 (A, B, D)-, α2 (A, B, C)- and β (1, 2, 3)-ARs mRNA and protein expression and the localization of these receptors in the porcine uterus. On Day 3 of the estrous cycle (Day 0 of the study), 50 ml of either saline (group SAL) or E. coli suspension (10 colony-forming units/ml, group E. coli) were injected into each uterine horn. In the control pigs (group CON), only laparotomy was performed. Eight days later, α1D-ARs mRNA (P < 0.001) and protein (P < 0.05) levels and α2A-ARs protein level (P < 0.05) were increased in the inflamed endometrium, while the α2C-ARs protein level (P < 0.001) was lowered, as compared to the SAL and CON groups. In the inflamed endometrium, β2-ARs mRNA (P < 0.01) and protein (CON: P < 0.01, SAL: P < 0.001) expression was lower than in the other two groups, and β1-ARs mRNA (P < 0.001) and protein (P < 0.01) expression was higher compared to the SAL group. After bacterial treatment, α2A- (P < 0.001) and α2B (P < 0.05) -ARs protein levels and β2-ARs mRNA (CON: P < 0.01, SAL: P < 0.05) and protein (CON: P < 0.01, SAL: P < 0.05) expression in myometrium were found to be increased compared to both groups. In turn, in myometrium following E. coli infusion, the α2C-ARs protein level was lower (P < 0.01) than in the CON group. All studied receptors were present in the luminal and glandular epithelium, blood vessels and myometrial muscular cells of the gilt uteri in the E. coli, SAL and CON groups. The data show that inflammation changes the ARs expression in porcine uterus, suggesting their importance in the course/consequences of uterine inflammation. Those affected ARs may constitute a therapeutic target in an inflamed uterus.
在生理条件下，去甲肾上腺素（NA）和肾上腺素能受体（ARs）与子宫功能有关。NA和ARs在炎症子宫中的作用尚未完全明确。本研究旨在确定炎症对猪子宫中α1（A、B、D）-、α2（A、B、C）-和β（1、2、3）-ARs mRNA和蛋白表达水平以及这些受体定位的影响。在发情周期的第3天（研究第0天），向每个子宫角注射50毫升生理盐水（SAL组）或大肠杆菌悬液（10个菌落形成单位/毫升，大肠杆菌组）。在对照猪（CON组）中，仅进行剖腹手术。八天后，与SAL组和CON组相比，炎症子宫内膜中α1D-ARs mRNA（P<0.001）和蛋白（P<0.05）水平以及α2A-ARs蛋白水平（P<0.05）升高，而α2C-ARs蛋白水平降低（P<0.001）。在炎症子宫内膜中，β2-ARs mRNA（P<0.01）和蛋白（CON组：P<0.01，SAL组：P<0.001）表达低于其他两组，β1-ARs mRNA（P<0.001）和蛋白（P<0.01）表达高于SAL组。细菌处理后，与两组相比，子宫肌层中α2A-（P<0.001）和α2B-ARs（P<0.05）蛋白水平以及β2-ARs mRNA（CON组：P<0.01，SAL组：P<0.05）和蛋白（CON组：P<0.01，SAL组：P<0.05）表达增加。反过来，在大肠杆菌注入后的子宫肌层中，α2C-ARs蛋白水平低于CON组（P<0.01）。在大肠杆菌组、SAL组和CON组的后备母猪子宫中，所有研究的受体均存在于腔上皮、腺上皮、血管和子宫肌层肌细胞中。数据表明，炎症改变了猪子宫中ARs的表达，提示它们在子宫炎症过程/后果中的重要性。那些受影响的ARs可能构成炎症子宫的治疗靶点。
