Division of Reproductive Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10 Str., 10-748, Olsztyn, Poland.
Department of Clinical Physiology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego Str. 13, 10-718, Olsztyn, Poland.
Sci Rep. 2021 Jun 1;11(1):11512. doi: 10.1038/s41598-021-91184-5.
Uterine inflammation is a very common and serious condition in domestic animals. To development and progression of this pathology often lead disturbances in myometrial contractility. Participation of β1-, β2- and β3-adrenergic receptors (ARs) in noradrenaline (NA)-influenced contractility of the pig inflamed uterus was studied. The gilts of SAL- and E.coli-treated groups were administered saline or E.coli suspension into the uterine horns, respectively. Laparotomy was only done in the CON group. Compared to the period before NA administration, this neurotransmitter reduced the tension, amplitude and frequency in uterine strips of the CON and SAL groups. In the E.coli group, NA decreased the amplitude and frequency, and these parameters were lower than in other groups. In the CON, SAL and E.coli groups, β1- and β3-ARs antagonists in more cases did not significantly change and partly eliminated NA inhibitory effect on amplitude and frequency, as compared to NA action alone. In turn, β2-ARs antagonist completely abolished NA relaxatory effect on these parameters in three groups. Summarizing, NA decreases the contractile amplitude and frequency of pig inflamed uterus via all β-ARs subtypes, however, β2-ARs have the greatest importance. Given this, pharmacological modulation of particular β-ARs subtypes can be used to increase inflamed uterus contractility.
子宫炎症是家畜中非常常见和严重的疾病。这种病理学的发展和进展通常导致子宫平滑肌收缩性紊乱。研究了β1-、β2-和β3-肾上腺素能受体（AR）在去甲肾上腺素（NA）影响猪发炎子宫收缩性中的参与。分别向 SAL 和大肠杆菌处理组的母猪子宫角中注入生理盐水或大肠杆菌悬浮液。仅在 CON 组中进行剖腹手术。与 NA 给药前相比，这种神经递质降低了 CON 和 SAL 组子宫带的张力、振幅和频率。在大肠杆菌组中，NA 降低了振幅和频率，这些参数低于其他组。在 CON、SAL 和大肠杆菌组中，β1-和β3-AR 拮抗剂在更多情况下并未显著改变并部分消除了 NA 对振幅和频率的抑制作用，与单独的 NA 作用相比。相反，β2-AR 拮抗剂完全消除了 NA 在三组中对这些参数的松弛作用。综上所述，NA 通过所有β-AR 亚型降低猪发炎子宫的收缩幅度和频率，但β2-AR 具有最大的重要性。鉴于此，特定β-AR 亚型的药理学调节可用于增加发炎子宫的收缩性。
