Division of Reproductive Biology, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Olsztyn, Poland.
Division of Clinical Physiology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland.
PLoS One. 2020 Jul 10;15(7):e0236044. doi: 10.1371/journal.pone.0236044. eCollection 2020.
In the experiment the influence of inflammation on neuropeptide Y (NPY) receptor subtype 1 (Y1Rs) and 2 (Y2Rs) expression pattern in the gilt myometrium and NPY effect alone or with Y1Rs and Y2Rs antagonists on contractility of an inflamed uterus were recognized. On Day 3 of the estrous cycle, either E.coli suspension (E.coli group) or saline (SAL group) were administered into uterine horns. In the control gilts (CON group), only laparotomy was carried out. Eight days later, E.coli treatment evoked severe acute endometritis, significantly reduced Y1Rs mRNA and protein expression and increased Y2Rs protein expression in myometrium in relation to the CON and SAL groups. Compared to period before NPY application, NPY (10-7 M) significantly reduced amplitude in myometrium and endometrium/myometrium and frequency in myometrium of the CON and SAL groups and amplitude in endometrium/myometrium and frequency in strips of the E.coli group. In this group, after using NPY (10-7 M), the amplitude rose significantly in both kinds of strips, while frequency fell in endometrium/myometrium in relation to the SAL group. In the CON and SAL groups, NPY (10-7 M) with Y1Rs antagonist significantly increased the amplitude in endometrium/myometrium, while with Y2Rs antagonist caused a rise in myometrium. In the E.coli group after using Y1Rs or Y2Rs antagonist and NPY (10-7 M) amplitude did not significantly change in endometrium/myometrium, and this parameter was significantly lower after using the NPY and Y1Rs antagonist than in other groups. Y1Rs antagonist and NPY (10-8, 10-7 M) significantly increased frequency in endometrium/myometrium of the CON and SAL groups. In the E.coli group, Y1Rs or Y2Rs antagonists and NPY (10-7 M) significantly increased frequency in myometrium and it was significantly higher than in the CON group. Inflammation changes Y1Rs and Y2Rs expression in myometrium of the inflamed pig uterus and NPY reduces this organ contractility by Y1Rs and Y2Rs.
在实验中，研究了炎症对母猪子宫神经肽 Y (NPY) 受体亚型 1 (Y1Rs) 和 2 (Y2Rs) 表达模式的影响，以及 NPY 单独或与 Y1Rs 和 Y2Rs 拮抗剂对炎症子宫收缩性的影响。在发情周期的第 3 天，将大肠杆菌悬浮液（大肠杆菌组）或生理盐水（SAL 组）注入子宫角。在对照组母猪（CON 组）中，仅进行剖腹手术。8 天后，大肠杆菌处理引发严重急性子宫内膜炎，与 CON 和 SAL 组相比，显著降低了子宫肌层中 Y1Rs mRNA 和蛋白的表达，并增加了 Y2Rs 蛋白的表达。与 NPY 应用前相比，NPY（10-7 M）显著降低了 CON 和 SAL 组子宫肌层和子宫内膜/子宫肌层的振幅以及子宫肌层的频率，也降低了大肠杆菌组子宫内膜/子宫肌层的振幅和条带的频率。在该组中，使用 NPY（10-7 M）后，两种类型的条带的振幅均显著升高，而与 SAL 组相比，子宫内膜/子宫肌层的频率下降。在 CON 和 SAL 组中，NPY（10-7 M）与 Y1Rs 拮抗剂一起使用显著增加了子宫内膜/子宫肌层的振幅，而与 Y2Rs 拮抗剂一起使用导致子宫肌层的振幅增加。在使用 Y1Rs 或 Y2Rs 拮抗剂和 NPY（10-7 M）后，大肠杆菌组的子宫内膜/子宫肌层的振幅没有显著变化，并且与其他组相比，使用 NPY 和 Y1Rs 拮抗剂后该参数显著降低。Y1Rs 拮抗剂和 NPY（10-8、10-7 M）显著增加了 CON 和 SAL 组子宫内膜/子宫肌层的频率。在大肠杆菌组中，Y1Rs 或 Y2Rs 拮抗剂和 NPY（10-7 M）显著增加了子宫肌层的频率，并且明显高于 CON 组。炎症改变了感染猪子宫中 Y1Rs 和 Y2Rs 的表达，NPY 通过 Y1Rs 和 Y2Rs 降低了该器官的收缩性。
