E蛋白的两种功能是质粒F复制控制系统中的关键要素。
Two functions of the E protein are key elements in the plasmid F replication control system.
作者信息
Rokeach L A, Søgaard-Andersen L, Molin S
出版信息
J Bacteriol. 1985 Dec;164(3):1262-70. doi: 10.1128/jb.164.3.1262-1270.1985.
Abstract
By using a plasmid carrying a translational fusion between the E gene of the IncFI plasmid F and the lacZ gene, we located the operator of the autogenously regulated E gene to an inverted repeat overlapping the E-gene promoter and showing perfect homology to part of the sequence found in all the direct repeats of two regions exerting an inhibitory effect on F replication, incB and incC. Excess E protein provided in trans to an F plasmid increased the replication frequency of the F plasmid. This stimulatory effect was counteracted by increased dosages of incB or incC. A model is proposed for the replication control system of F in which the key elements are autoregulation of E-gene expression and titration of E protein by incB and incC.
摘要
通过使用一个携带IncFI质粒F的E基因与lacZ基因之间翻译融合的质粒，我们将自主调节的E基因的操纵子定位到一个与E基因启动子重叠的反向重复序列上，该序列与在对F复制发挥抑制作用的两个区域（incB和incC）的所有直接重复序列中发现的部分序列具有完美的同源性。反式提供给F质粒的过量E蛋白增加了F质粒的复制频率。这种刺激作用被增加剂量的incB或incC抵消。提出了一个F复制控制系统的模型，其中关键要素是E基因表达的自动调节以及incB和incC对E蛋白的滴定作用。
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