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F'编码的温度敏感型λ cI857阻遏基因，用于λ启动子依赖性表达系统的简便构建与调控。

F'-coded, temperature-sensitive lambda cI857 repressor gene for easy construction and regulation of lambda promoter-dependent expression systems.

作者信息

Mieschendahl M, Müller-Hill B

出版信息

J Bacteriol. 1985 Dec;164(3):1366-9. doi: 10.1128/jb.164.3.1366-1369.1985.

DOI:10.1128/jb.164.3.1366-1369.1985
PMID:2999084
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC219341/
Abstract

We describe the construction and properties of an F' factor which carries the temperature-sensitive cI857 allele of the repressor gene of coliphage lambda and which lacks the lambda cro function. This episome can easily be transferred to any F- and F' Escherichia coli strain, thus facilitating the construction and regulation of lambda promoter-dependent expression systems without the use of defective prophages.

摘要

我们描述了一种F'因子的构建及其特性，该因子携带噬菌体λ阻遏基因的温度敏感型cI857等位基因，且缺乏λ cro功能。这种附加体可以很容易地转移到任何F-和F'大肠杆菌菌株中，从而便于构建和调控依赖λ启动子的表达系统，而无需使用缺陷型原噬菌体。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/303c/219341/8751f60941e7/jbacter00217-0402-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/303c/219341/8751f60941e7/jbacter00217-0402-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/303c/219341/8751f60941e7/jbacter00217-0402-a.jpg

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F'-coded, temperature-sensitive lambda cI857 repressor gene for easy construction and regulation of lambda promoter-dependent expression systems.F'编码的温度敏感型λ cI857阻遏基因，用于λ启动子依赖性表达系统的简便构建与调控。
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本文引用的文献

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THE PROPERTIES OF REPRESSOR AND THE KINETICS OF ITS ACTION.阻遏物的特性及其作用动力学
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Overproduction of proteins in recombinant organisms.
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Nucleotide sequence of the promoter region of the citrate synthase gene (gltA) of Escherichia coli.大肠杆菌柠檬酸合酶基因（gltA）启动子区域的核苷酸序列。
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Transcription from efficient promoters can interfere with plasmid replication and diminish expression of plasmid specified genes.来自高效启动子的转录可能会干扰质粒复制并降低质粒特定基因的表达。
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The use of pKc30 and its derivatives for controlled expression of genes.pKc30及其衍生物在基因的可控表达中的应用。
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