通过在下游放置RNA终止信号，使得强启动子的克隆和分析成为可能。
Cloning and analysis of strong promoters is made possible by the downstream placement of a RNA termination signal.
作者信息
Gentz R, Langner A, Chang A C, Cohen S N, Bujard H
出版信息
Proc Natl Acad Sci U S A. 1981 Aug;78(8):4936-40. doi: 10.1073/pnas.78.8.4936.
Abstract
Downstream placement of a strong transcriptional termination signal has made possible the cloning of bacteriophage T5 promoters known to exhibit high signal strength. The cloning system constructed contains two easily assayable indicator functions whose expression is controlled by the integration of promoters and terminators, respectively. By assessing transcription within the indicator regions, the efficiency of promoters as well as termination signals can be determined in vitro and in vivo.
摘要
在强转录终止信号的下游进行放置，使得克隆已知具有高信号强度的噬菌体T5启动子成为可能。构建的克隆系统包含两个易于检测的指示功能，其表达分别由启动子和终止子的整合来控制。通过评估指示区域内的转录，可以在体外和体内确定启动子以及终止信号的效率。
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